中国免疫学杂志2026，Vol.42Issue(8)：1803-1808,6.DOI:10.3969/j.issn.1000-484X.2026.08.002
LncRNA GAS5靶向调控miR-29a-3p对肺炎链球菌诱导的人肺泡上皮细胞增殖、凋亡的影响
Effects of LncRNA GAS5 on proliferation and apoptosis of human alveolar epithelial cells induced by Streptococcus pneumoniae through targeted regulation of miR-29a-3p
摘要
Abstract
Objective:To investigate the effects of LncRNA GAS5 on the proliferation and apoptosis of human alveolar epithe-lial cells induced by Streptococcus pneumoniae through targeted regulation of miR-29a-3p.Methods:When A549 cells reached 80%confluence,experimental groups were performed:Control group(normal culture),infection(1×108 CFU/mL Streptococcus pneumoniae incubation)group,infection+negative control(pcDNA)group,infection+GAS5 overexpression(pcDNA GAS5)group,infection+miR-29a-3p inhibitor(inhibitor)group,infection+negative control(inhibitor NC)group,infection+pcDNA GAS5+negative control(miR NC)group,and infection+pcDNA GAS5+miR-29a-3p group.The above plasmids or oligonucleotides were transfected into A549 cells using liposome transfection method,and then incubated with 1×108 CFU/mL Streptococcus pneumoniae for 48 hours.Subsequently,GAS5,miR-29a-3p expression,cell proliferation and related protein(Ki-67,PCNA)expression,apoptosis and related protein(Bax,Bcl-2)expression,inflammatory cytokines IL-6,IL-1β,IL-10 levels in the supernatant,and the targeting relationship between GAS5 and miR-29a-3p were detected.Results:Compared with the control group,the expression of GAS5,proliferation rate,IL-10 level,Ki67,PCNA and Bcl-2 in the infection group were obviously lower,while miR-29a-3p,apoptosis rate,IL-6,IL-1β level,and Bax expression were obviously higher(P<0.05).Compared with the infection+pcDNA group,the GAS5 expression,proliferation rate,IL-10 level,Ki67,PCNA and Bcl-2 expressions were obviously higher in the infection+pcDNA GAS5 group,but miR-29a-3p,apoptosis rate,IL-6,IL-1β level,and Bax expression were obviously lower(P<0.05).Compared with the infection+inhibitor NC group,the pro-liferation rate,IL-10 level,Ki67,PCNA and Bcl-2 expressions were obviously higher in the infection+miR-29a-3p inhibitor group.However,miR-29a-3p,apoptosis rate,IL-6,IL-1β levels and Bax expression were obviously lower(P<0.05),while GAS5 expres-sion showed no obvious difference(P>0.05).Compared with the infection+pcDNA GAS5+miR NC group,the proliferation rate,IL-10 level,Ki67,PCNA and Bcl-2 expressions in the infection+pcDNA GAS5+miR-29a-3p group were obviously lower,but miR-29a-3p,apoptosis rate,IL-6,IL-1β levels and Bax expression were obviously higher(P<0.05),while there was no obvious difference in GAS5 expression(P>0.05).GAS5 and miR-29a-3p had a negative targeted regulatory relationship.Conclusion:Up-regulation of Ln-cRNA GAS5 reduces the proliferation and promotes apoptosis of human alveolar epithelial cells induced by Streptococcus pneumoniae by targeting miR-29a-3p.关键词
LncRNA GAS5/miR-29a-3p/肺炎链球菌/人肺泡上皮细胞/增殖/凋亡Key words
LncRNA GAS5/miR-29a-3p/Streptococcus pneumoniae/Human alveolar epithelial cells/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
李群,蔡明丹,张萍萍,黄志心,陈海燕,谢丛华..LncRNA GAS5靶向调控miR-29a-3p对肺炎链球菌诱导的人肺泡上皮细胞增殖、凋亡的影响[J].中国免疫学杂志,2026,42(8):1803-1808,6.基金项目
湖北省卫生健康委员会联合基金项目(WJ2019H002). （WJ2019H002）
