中国免疫学杂志2026，Vol.42Issue(8)：1834-1839,1846,7.DOI:10.3969/j.issn.1000-484X.2026.08.007
右美托咪定调节SIRT1/PGC-1α/Nrf2信号通路对IL-1β诱导的软骨细胞损伤的影响
Effect of dexmedetomidine on IL-1β-induced chondrocyte injury by modulating SIRT1/PGC-1α/Nrf2 signaling pathway
摘要
Abstract
Objective:To investigate the effect of dexmedetomidine(DEX)on IL-1β-induced chondrocyte injury by modulating silent information regulator 1(SIRT1)/PPARγ coactivator factor-1α(PGC-1α)/nuclear factor E2 related factor 2(Nrf2)signaling pathway.Methods:Rat chondrocytes were isolated and cultured in vitro,toluidine blue staining and immunofluorescence were used to identify chondrocytes.Chondrocytes were co-treated with DEX at concentrations ranging from 3.125 to 200 nmol/L and IL-1β.CCK-8 method was used to detect cell viability and screen for the optimal concentration of DEX.Chondrocytes were assigned into Control group,IL-1β group,DEX low,medium and high concentration groups(DEX-L group,DEX-M group,DEX-H group)and DEX high concentration+SIRT1 inhibitor group(DEX-H+EX527 group).Cell apoptosis was detected by flow cytometry.Levels of cellular inflam-matory factors(IL-6,TNF-α,IFN-γ)and oxidative stress(ROS,MDA)were measured by kits.Western blot was used to measure expressions of apoptotic proteins(Bax,Caspase-1),extracellular matrix related proteins(MMP-13,MMP3,Collagen Ⅱ),and path-way proteins.Results:Compared with Control group,apoptosis rate,Caspase-3,Bax,MMP13,MMP3 expression levels,IL-6,TNF-α,IFN-γ,ROS and MDA levels were increased in IL-1β group,while Collagen Ⅱ,SIRT1,PGC-1α,Nrf2,Nrf1,p-AMPK/AMPK and TFAM expression levels were decreased(P<0.05).Compared with IL-1β group,apoptosis rate,Caspase-3,Bax,MMP13,MMP3 expression levels,IL-6,TNF-α,IFN-γ,ROS and MDA levels were decreased in DEX-L,DEX-M,and DEX-H groups,while the Collagen Ⅱ,SIRT1,PGC-1α,Nrf2,Nrf1,p-AMPK/AMPK and TFAM expression levels were increased(P<0.05).Compared with DEX-H group,apoptosis rate,Caspase-3,Bax,MMP13,MMP3 expression levels,IL-6,TNF-α,IFN-γ,ROS and MDA levels were increased in DEX-H+EX527 group,while Collagen Ⅱ,SIRT1,PGC-1α,Nrf2,Nrf1,p-AMPK/AMPK and TFAM expression levels were decreased(P<0.05).Conclusion:DEX alleviates IL-1β-induced chondrocyte injury by activating the SIRT1/PGC-1α/Nrf2 signaling pathway.关键词
右美托咪定/SIRT1/PGC-1α/Nrf2信号通路/IL-1β诱导/软骨细胞损伤Key words
Dexmedetomidine/SIRT1/PGC-1α/Nrf2 signaling pathway/IL-1β induction/Chondrocyte injury分类
医药卫生引用本文复制引用
刘洪芳,杨猛,刘希明..右美托咪定调节SIRT1/PGC-1α/Nrf2信号通路对IL-1β诱导的软骨细胞损伤的影响[J].中国免疫学杂志,2026,42(8):1834-1839,1846,7.基金项目
徐州医科大学附属医院资助项目(XYFM202422). （XYFM202422）
