中国肿瘤生物治疗杂志2026，Vol.33Issue(7)：731-736,6.DOI:10.3872/j.issn.1007-385X.2026.07.003
甜橙黄酮通过调节Fas/FasL信号通路影响子宫内膜癌细胞的恶性生物学行为
Sinensetin affects malignant biological behaviors of endometrial cancer cells by regulating the Fas/FasL signaling pathway
摘要
Abstract
Objective:To investigate the effect of sinensetin on malignant biological behaviors of endometrial cancer cells through regulation of the Fas/FasL signaling pathway.Methods:Human endometrial cancer cell lines Ishikawa(ISK),RL95-2,AN3-CA,and KLE were selected as research subjects.The CCK-8 assay was initially used to screen the inhibitory effect of sinensetin on the proliferation of these four cell lines(treatment with 120 μmol/L for 48 h).Based on this,ISK cells,which were more sensitive to sinensetin,were selected for subsequent experiments and divided into the following groups:control group,L-sinensetin group(40 μmol/L),M-sinensetin group(80 μmol/L),H-sinensetin group(120 μmol/L),H-sinensetin+si-NC group,and H-sinensetin+si-Fas group.The CCK-8 assay,colony formation assay,Transwell assay,and flow cytometry were used to detect cell proliferation,invasion,and apoptosis,respectively.Vasculogenic mimicry assay was performed to assess the vasculogenic mimicry-forming ability of cells in each group,while WB was employed to detect the protein expression levels of Fas,FasL,and cleaved caspase-8 in each group.Results:Sinensetin inhibited the proliferation of endometrial cancer cell lines ISK,RL95-2,AN3-CA,and KLE(all P<0.01),with the most pronounced effect observed in ISK cells.In ISK cells,compared with the control group,the levels of cell proliferation,number of colonies,number of invading cells,and number of vasculogenic mimicry branches in the L-sinensetin group,M-sinensetin group,and H-sinensetin group gradually decreased,while the apoptosis rate and the protein expression levels of Fas,FasL,and cleaved caspase-8 gradually increased.Moreover,higher concentrations of sinensetin were associated with more pronounced changes in the above indicators(P<0.01).Compared with the H-sinensetin+si-NC group,the H-sinensetin+si-Fas group showed increased cell proliferation,number of colonies,number of invading cells,and number of vasculogenic mimicry branches,as well as decreased apoptosis rate and reduced protein expression levels of Fas,FasL,and cleaved caspase-8 in ISK cells(P<0.01).Conclusion:Sinensetin inhibits proliferation,invasion,and vasculogenic mimicry of endometrial cancer cells and induces apoptosis,which may be associated with the activation of the Fas/FasL signaling pathway.关键词
子宫内膜癌/甜橙黄酮/Fas/FasL信号通路/Ishikawa/恶性生物学行为Key words
endometrial cancer/sinensetin/Fas/FasL signaling pathway/Ishikawa(ISK)/malignant biological behavior分类
医药卫生引用本文复制引用
李兴艳,陈林兴,何丹,彭强丽,胡泽蓉,周瑞彬..甜橙黄酮通过调节Fas/FasL信号通路影响子宫内膜癌细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志,2026,33(7):731-736,6.基金项目
云南省科技厅科技计划项目(202101AZ070001-031) （202101AZ070001-031）
