中国肿瘤生物治疗杂志2026，Vol.33Issue(7)：737-745,9.DOI:10.3872/j.issn.1007-385X.2026.07.004
COA6在胃癌中的表达及其功能作用:通过AMPK/mTOR信号通路调控恶性生物学行为
Expression and functional role of COA6 in gastric cancer:regulation of malignant behaviors via the AMPK/mTOR signaling pathway
摘要
Abstract
Objective:To investigate the expression characteristics and clinical significance of cytochrome c oxidase assembly factor 6(COA6)in gastric cancer,as well as its regulatory effects and molecular mechanisms on the malignant biological behaviors of gastric cancer cells.Methods:The TIMER 2.0,The Human Protein Atlas(HPA),and UALCAN public databases were utilized to analyze the mRNA expression levels and partial protein expression information of COA6 in pan-cancer tissues,and to assess its association with clinicopathological features.Lentiviral vectors carrying COA6 knockdown and overexpression sequences were constructed and transfected into human gastric cancer SGC-7901 and MGC-803 cell lines to establish stably modified COA6 cell models.For the knockdown experiment,three experimental groups(shCOA6-1,shCOA6-2,shCOA6-3)and a negative control group(shCOA6-NC)were established;for the overexpression experiment,an OE group(COA6 overexpression)and a Vector group(empty lentivirus control)were set up.WB was performed to verify the efficiencies of COA6 knockdown and overexpression in each group.Subsequently,cell proliferative activity was determined via cell counting assay,cell invasive capacity was evaluated using Transwell assay,and cell migratory ability was examined by wound healing assay.Annexin V-FITC/PI double staining combined with flow cytometry was adopted to quantify cellular apoptosis,and WB was also used to detect the phosphorylation levels of core proteins in the AMPK/mTOR signaling pathway.Results:COA6 was aberrantly expressed in multiple cancers,and its expression level was associated with clinical stage,pathological grade,lymph node metastasis,and TP53 mutation status in gastric cancer.Functionally,COA6 knockdown inhibited malignant phenotypes and promoted apoptosis(all P<0.000 1),while overexpression had opposite effects.Mechanistically,COA6 knockdown increased p-AMPK levels and decreased p-mTOR levels.This effect was reversed by treatment with the AMPK inhibitor Compound C,which abrogated the COA6 knockdown-induced increase in p-AMPK and decrease in p-mTOR.Conclusion:COA6 is highly expressed in gastric cancer and is associated with several clinicopathological features.It may promote proliferation,invasion,and migration,while inhibiting apoptosis of gastric cancer cells by regulating the AMPK/mTOR signaling pathway.关键词
细胞色素c氧化酶组装因子6/胃癌/SGC-7901/MGC-803/AMPK/mTOR信号通路/恶性生物学行为Key words
cytochrome c oxidase assembly factor 6(COA6)/gastric cancer/SGC-7901/MGC-803/AMPK/mTOR signaling pathway/malignant biological behavior分类
医药卫生引用本文复制引用
杨丹,熊心芽,孙杭杭,韩然..COA6在胃癌中的表达及其功能作用:通过AMPK/mTOR信号通路调控恶性生物学行为[J].中国肿瘤生物治疗杂志,2026,33(7):737-745,9.基金项目
国家自然科学基金(81960536) （81960536）
贵州省科技厅项目(黔科合基础[2020]1Y379) （黔科合基础[2020]1Y379）
