中国肿瘤生物治疗杂志2026，Vol.33Issue(7)：746-753,8.DOI:10.3872/j.issn.1007-385X.2026.07.005
姜黄素及其代谢产物四氢姜黄素对乳腺癌MCF-7/ADR细胞多柔比星耐药的逆转作用及其可能的机制
Curcumin and its metabolite tetrahydrocurcumin reverse doxorubicin resistance in MCF-7/ADR breast cancer cells:possible mechanisms
摘要
Abstract
Objective:To investigate the effects of curcumin and its metabolite tetrahydrocurcumin on reversing doxorubicin resistance in MCF-7/ADR breast cancer cells and to explore the possible mechanisms.Methods:The inhibitory effects of curcumin and tetrahydrocurcumin on MCF-7/ADR cell proliferation and their effects on the reversal of doxorubicin resistance were assessed using the CCK-8 assay.Cell migration and invasion were evaluated using wound-healing and Transwell assays,respectively.Apoptosis was analyzed by flow cytometry.The mRNA levels of glutathione S-transferase pi 1(GSTP1),Janus kinase 1(JAK1),and signal transducer and activator of transcription 3(STAT3),as well as the protein levels of GSTP1,JAK1,phosphorylated JAK1(p-JAK1),STAT3,and phosphorylated STAT3(p-STAT3),were measured by quantitative real-time PCR and WB assay,respectively.Results:Both curcumin and tetrahydrocurcumin inhibited MCF-7/ADR cell proliferation in a concentration-dependent manner.The IC₅₀ of tetrahydrocurcumin(18.62±2.15)μmol/L was significantly lower than that of curcumin(37.45±3.28)μmol/L(P<0.05).Both compounds enhanced the inhibitory effect of doxorubicin on MCF-7/ADR cell proliferation,and the resistance reversal index of tetrahydrocurcumin(4.82)was significantly higher than that of curcumin(2.96)(P<0.05).Compared with the 0 μmol/L groups,both compounds inhibited MCF-7/ADR cell migration and invasion in a concentration-dependent manner,promoted apoptosis,and downregulated GSTP1,JAK1,and STAT3 mRNA expression as well as GSTP1,p-JAK1,and p-STAT3 protein expression(all P<0.05).Treatment with 80 μmol/L curcumin or tetrahydrocurcumin significantly reduced the wound-healing rate and the number of invading cells and significantly increased the apoptosis rate(all P<0.05).Conclusion:Curcumin and its metabolite tetrahydrocurcumin can reverse doxorubicin resistance in MCF-7/ADR breast cancer cells,possibly by inhibiting cell migration and invasion,promoting apoptosis,downregulating GSTP1 expression,and suppressing the phosphorylation of JAK1 and STAT3.关键词
谷胱甘肽S-转移酶π-1/JAK1/STAT3信号通路/姜黄素/四氢姜黄素/乳腺癌/耐药性/多柔比星Key words
glutathione S-transferase pi 1(GSTP1)/JAK1/STAT3 signaling pathway/curcumin/tetrahydrocurcumin/breast cancer/drug resistance/doxorubicin分类
医药卫生引用本文复制引用
孙小虎,王蕴华,刘燕,孟然,曹旭晨..姜黄素及其代谢产物四氢姜黄素对乳腺癌MCF-7/ADR细胞多柔比星耐药的逆转作用及其可能的机制[J].中国肿瘤生物治疗杂志,2026,33(7):746-753,8.基金项目
国家自然科学基金(82274221) （82274221）
天津市医学重点学科(专科)建设项目(TJYXZDXK-009A) （专科）
