中国沼气2026，Vol.44Issue(4)：18-26,9.DOI:10.20022/j.cnki.1000-1166.2026040018
基于酶解-丙三醇发酵预处理的纤维素厌氧发酵工艺探究
Study on Process of Anaerobic Digestion of Cellulose Based on Enzymatic Hydrolysis-Glycerol Fermentation Pre-treatment
摘要
Abstract
Direct inter-species electron transfer(DIET)for methane production eliminates the need for hydrolysis and acid-ification,and electron transfer does not depend on H2 diffusion.This approach overcomes the rate constraints of cellulose hy-drolysis in anaerobic fermentation and the thermodynamic barriers to organic decomposition for acetate production.The addi-tion of glycerol can establish DIET in anaerobic fermentation systems,enhancing methane production efficiency.However,the addition of glycerol requires large doses and incurs high costs.Therefore,this study proposes an anaerobic cellulose fer-mentation process based on enzymatic hydrolysis-glycerol fermentation pretreatment.Results indicate:1)Optimal enzyme loading and cycle for cellulose hydrolysis are 10％and 2 d;optimal temperature,inoculum ratio,and cycle for glycerol fer-mentation pretreatment are 32 ℃,15％,and 9 days.2)The methane production rate in the enzyme-glycerol fermentation pretreatment group reached 1.9 m3·m-3d-1),a 90％increase compared to the untreated group;the organic matter conver-sion efficiency to methane reached 95 L·kg-1VSd-1,a 74％improvement over the untreated group.3)The microbial community in the enzymatic hydrolysis-glycerol fermentation pretreatment group exhibited higher conductivity,greater capacitance,and lower internal resistance.Sphaerochaeta globosa and Methanobacterium formicicum were significantly enriched.关键词
纤维素/厌氧发酵/直接种间电子传递/酶解/丙三醇发酵Key words
cellulose/anaerobic fermentation/direct interspecific electron transfer/enzymatic hydrolysis/glycerol Fer-mentation分类
农业科技引用本文复制引用
曾茂鑫,王珍如,李媛,赵智强..基于酶解-丙三醇发酵预处理的纤维素厌氧发酵工艺探究[J].中国沼气,2026,44(4):18-26,9.基金项目
国家重点研发计划青年科学家项目(2022YFA0913100) （2022YFA0913100）
国家自然科学基金面上项目(52470140) （52470140）
"兴辽英才计划"项目(XLYC2503144). （XLYC2503144）
