中国中药杂志2026，Vol.51Issue(11)：3039-3048,10.DOI:10.19540/j.cnki.cjcmm.20260130.401
麦味养肺汤通过p53信号通路抑制细胞衰老改善小鼠肺纤维化
Maiwei Yangfei Decoction alleviates pulmonary fibrosis in mice by inhibiting cellular senescence via p53 signaling pathway
摘要
Abstract
This study aimed to investigate the therapeutic effect of Maiwei Yangfei Decoction(MWYF)on pulmonary fibrosis and its underlying mechanisms.Seventy-two C57BL/6J mice were randomly divided into a blank group,a model group,a low-dose MWYF group,a medium-dose MWYF group,a high-dose MWYF group,and a pirfenidone group.A pulmonary fibrosis model was established by intratracheal instillation of bleomycin.The blank group and the model group were administered double-distilled water daily,while the MWYF groups received corresponding concentrations of MWYF,and the positive control group was given pirfenidone.The severity of pulmonary fibrosis was assessed through Micro-CT scans,histopathological staining of lung tissues,and the expression of collagen Ⅰ and collagen Ⅲ.Network pharmacology combined with transcriptomics analysis was used to explore the anti-fibrotic mechanism of MWYF,which was further validated through in vivo experiments.The results showed that MWYF reduced pulmonary fibrotic lesions,improved inflammation and fibrosis scores,and downregulated the protein expression of collagen Ⅰ and collagen Ⅲ.Network pharmacology and transcriptomics suggested that the anti-fibrotic effect of MWYF was associated with cellular senescence and the p53 signaling pathway.In vivo experiments demonstrated that MWYF significantly reduced senescence-associated β-galactosidase(SA-β-Gal)activity in lung tissues,downregulated the expression of key senescence-related proteins p53,p21,and p16,and decreased cellular senescence,with the high-dose group showing the most pronounced effects.Collectively,these findings demonstrated that MWYF attenuated bleomycin-induced pulmonary fibrosis in mice,likely by suppressing cellular senescence via modulation of the p53 signaling pathway.关键词
肺纤维化/麦味养肺汤/p53/细胞衰老Key words
pulmonary fibrosis/Maiwei Yangfei Decoction/p53/cellular senescence引用本文复制引用
柏乐,赵琪,朱栋伟,潘婷钰,李叶卉,武琦,徐泳,周贤梅..麦味养肺汤通过p53信号通路抑制细胞衰老改善小鼠肺纤维化[J].中国中药杂志,2026,51(11):3039-3048,10.基金项目
国家自然科学基金项目(82505512,82074358) （82505512,82074358）
江苏省自然科学基金项目(BK20240731) （BK20240731）
徐州医科大学-齐鲁临床研究基金项目(QL-YB056) （QL-YB056）
