中国中药杂志2026，Vol.51Issue(11)：3060-3066,7.DOI:10.19540/j.cnki.cjcmm.20260320.701
黄芩素下调TGF-β1/Smad3信号改善肺泡上皮细胞衰老的机制研究
Mechanism research of baicalein in improving alveolar epithelial cell senescence via downregulating TGF-β1/Smad3 signal
摘要
Abstract
This study aims to investigate the effect and mechanism of baicalein in improving alveolar epithelial cell senescence.A cellular model of alveolar epithelial cell senescence induced by transforming growth factor-β1(TGF-β1)was established.Cells were randomly divided into control group,model group,baicalein group,small interfering RNA(siRNA)targeting Smad family member 2(Smad2)group,siRNA targeting Smad3 group,and baicalein+Smad3 plasmid group.Western blot was employed to detect the protein expression level of plasminogen activator inhibitor-1(PAI-1),a marker protein of alveolar epithelial cell senescence.Senescence-associated β-galactosidase(SA-β-gal)staining was used to measure the percentage of SA-β-gal-positive cells.The results show that baicalein can significantly inhibit the protein expression of PAI-1 in TGF-β1-induced alveolar epithelial cells and markedly reduce the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells.Baicalein can also significantly decrease the phosphorylation levels of Smad2 protein and Smad3 protein in TGF-β1-induced alveolar epithelial cells.Knockdown of Smad2 does not affect the protein expression level of PAI-1 in TGF-β1-induced alveolar epithelial cells,nor does it alter the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells.In contrast,knockdown of Smad3 can significantly downregulate the protein expression level of PAI-1 and reduce the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells.Overexpression of Smad3 can notably reverse the inhibitory effects of baicalein on PAI-1 protein expression and the percentage of SA-β-gal-positive cells in TGF-β1-induced alveolar epithelial cells.In conclusion,baicalein improves alveolar epithelial cell senescence by downregulating the TGF-β1/Smad3 signaling,but this effect might be independent of Smad2 signaling.关键词
肺纤维化/黄芩素/肺泡上皮细胞衰老/转化生长因子-β1/纤溶酶原激活物抑制剂-1/Smad家族成员3Key words
pulmonary fibrosis/baicalein/alveolar epithelial cell senescence/transforming growth factor-β1/plasminogen activator inhibitor-1/Smad family member 3引用本文复制引用
顾嘉旭,张津玮,蒋小岗..黄芩素下调TGF-β1/Smad3信号改善肺泡上皮细胞衰老的机制研究[J].中国中药杂志,2026,51(11):3060-3066,7.基金项目
国家自然科学基金项目(82273991) （82273991）
