中国中药杂志2026，Vol.51Issue(13)：3707-3715,9.DOI:10.19540/j.cnki.cjcmm.20260316.201
山豆根中异戊烯基黄酮类成分及其细胞毒活性研究
Prenylated flavonoids from Sophorae Tonkinensis Radix et Rhizoma and their cytotoxic activities
摘要
Abstract
Comprehensive chromatographic separation methods were employed to isolate and purify the chemical constituents from the non-alkaloidal fraction of Sophorae Tonkinensis Radix et Rhizoma,and the structures of the compounds were identified with mass spectrometry(MS)and nuclear magnetic resonance(NMR)data.Fourteen prenylated flavonoids were isolated and identified,namely 5-isopentenylsophotonin Ⅰ(1),(2S)-6-isopentenyl-2'-hydroxyglabrol(2),sophoradin(3),sophoradochromene(4),1-prenylpterocarpin(5),sophoranone(6),glabrol(7),7,4'-dihydroxy-6,8-diprenyldihydroflavone(8),isobavachin(9),sophoranochromene(10),5-dehydroxylupinifolin(11),2-[{2'-(1-hydroxy-1-methylethyl)-7'-(3-methyl-2-butenyl)-2',3'-dihydrobenzofuran}-5'-yl]-7-hydroxy-8-(3-methyl-2-butenyl)chroman-4-one(12),ebenosin(13),and wighteone(14).Among them,compounds 1 and 2 were identified as new compounds,the 13 C-NMR data of compounds 3 and 4 were reported for the first time,and compounds 5 and 9 were isolated from this plant for the first time.The MTT assay was used to evaluate the cytotoxic activities of the isolates,and compounds 4,7,and 10 exhibited significant potent cytotoxic activities on HCT116 cells,with IC50 values ranging from 11.44 to 13.43 μmol·L-1,comparable with the positive control 5-fluorouracil(5-FU,IC50 value is 11.70 μmol·L-1).关键词
豆科/山豆根/异戊烯基黄酮/细胞毒活性Key words
Fabaceae/Sophorae Tonkinensis Radix et Rhizoma/prenylated flavonoids/cytotoxic activity引用本文复制引用
汤凤娇,尹静慧,邹玮,龙芳,莫小小,谭钦刚..山豆根中异戊烯基黄酮类成分及其细胞毒活性研究[J].中国中药杂志,2026,51(13):3707-3715,9.基金项目
国家自然科学基金项目(82060783) （82060783）
国家级大学生创新训练项目(202410601032) （202410601032）
桂林医科大学硕士研究生科研项目(GYYK2025019) （GYYK2025019）
