中国中药杂志2026，Vol.51Issue(14)：3997-4007,11.DOI:10.19540/j.cnki.cjcmm.20260424.301
载丹参酮ⅡA磺酸钠及丹酚酸B的活性氧响应性水凝胶抑制兔耳增生性瘢痕作用研究
Study on effect of ROS-responsive hydrogel containing sodium tanshinone ⅡA sulfonate and salvianolic acid B on inhibiting hypertrophic scars in rabbit ears
摘要
Abstract
This paper constructed a reactive oxygen species(ROS)-responsive hydrogel loaded with sodium tanshinone ⅡA sulfonate(STS)and salvianolic acid B(SAB)and evaluated its therapeutic effect on hypertrophic scars.A hydrogel was prepared by using hyaluronic acid(HA)and polyvinyl alcohol(PVA)bridged by phenylboronic acid(PBA)as the matrix with two drugs physically encapsulated inside.Its structure,rheological properties,and drug release behavior were characterized.Scar models of rabbit ears were established and divided into a blank control group,a model group,an asiaticoside cream group,a regular drug-loaded hydrogel group,and a drug-loaded ROS-responsive hydrogel group.After 21 days of intervention,the net increased thickness of the car was detected.The tissue morphology was detected by using hematoxylin-eosin and Masson staining.The expressions of α-smooth muscle actin(α-SMA)and collagen-Ⅰ(COL-Ⅰ)were detected by immunohistochemistry,and the levels of transforming growth factor-β1(TGF-β1),tissue inhibitor of metalloproteinases-1(TIMP-1),matrix metallopeptidase-9(MMP-9),ROS,and 8-hydroxy-2'-deoxyguanosine(8-OHdG)were assessed.The results show that,compared to those in the model group,the net increased thickness of scar,TGF-β1,TIMP-1,ROS,8-OHdG,α-SMA,and COL-Ⅰ in each treatment group were significantly reduced(P＜0.05),while MMP-9 levels were increased(P＜0.05),and the improvement effect in the drug-loaded ROS-responsive hydrogel group was better than that in the regular drug-loaded hydrogel group(P＜0.05).These findings indicate that the hydrogel can achieve smart drug release and significantly inhibit scar formation,providing a new strategy for treatment.关键词
增生性瘢痕/ROS响应性水凝胶/丹酚酸B/丹参酮ⅡA磺酸钠/抗瘢痕/药物递送系统/兔耳增生性瘢痕模型Key words
hypertrophic scar/ROS-responsive hydrogel/salvianolic acid B/sodium tanshinone ⅡA sulfonate/anti-scar/drug de-livery system/hypertrophic scar model of rabbit ears引用本文复制引用
许警航,彭睿翔,陈求芳,禹佳惠,庄泽槟,时军..载丹参酮ⅡA磺酸钠及丹酚酸B的活性氧响应性水凝胶抑制兔耳增生性瘢痕作用研究[J].中国中药杂志,2026,51(14):3997-4007,11.基金项目
国家自然科学基金项目(82173982) （82173982）
广东省自然科学基金项目(2022A1515011382) （2022A1515011382）
