中国中药杂志2026，Vol.51Issue(15)：4311-4320,10.DOI:10.19540/j.cnki.cjcmm.20260403.401
基于表型性状与SNP分子标记的山西连翘种质资源遗传多样性分析
Genetic diversity analysis of Forsythia suspensa germplasm resources in Shanxi based on phenotypic traits and SNP molecular markers
摘要
Abstract
This study aimed to clarify the degree of fruit phenotypic variation and the characteristics of genetic diversity,population structure,and genetic differentiation of Forsythia suspensa resources in Shanxi,providing an important basis for germplasm conservation and breeding of superior varieties.A total of 46 F.suspensa fruits were collected,and 12 agronomic traits were measured and analyzed.The population genetic structure and genetic diversity of F.suspensa germplasm were evaluated using simplified genome sequencing technology.For the five quality traits of the 46 fruits,the Shannon-Wiener index ranged from 0.631 to 1.074,and the Simpson index ranged from 0.379 to 0.560.The seven quantitative traits exhibited abundant genetic variation,with coefficients of variation ranging from 9.764%(fruit shape index)to 45.494%(forsythin content).Principal component analysis reduced the 12 phenotypic traits to four factors,with a cumulative variance contribution of 74.547%.Sequencing data showed mean Q20 and Q30 values of 98.13%and 94.33%,respectively,with an average GC content of 35.95%.After filtering,a total of 12 347 327 high-quality single nucleotide polymorphism(SNP)loci were obtained.Based on these high-quality SNPs,principal component analysis,population structure analysis,and phylogenetic tree construction were carried out.The 46 germplasm resources were divided into four groups;however,grouping showed little relationship with geographic origin,and intermixing occurred among regions.Mantel test revealed a significant but weak positive correlation between phenotypic and genetic distances(r=0.159,P=0.001).At the molecular level,the four groups exhibited moderate genetic diversity overall,and the genetic differentiation index among populations ranged from 0.027 to 0.084,indicating low to moderate differentiation.The rich genetic diversity of the main phenotypic traits provides a solid material basis for screening superior germplasm and genetic breeding of F.suspensa.关键词
种质资源/表型鉴定/SNP/简化基因组测序/连翘/遗传多样性分析Key words
germplasm resources/phenotypic identification/SNP/simplified genome sequencing/Forsythia suspensa/genetic di-versity analysis引用本文复制引用
牛富婷,裴帅帅,王俊斌,李骁,刘聪,高雪伊,郭淑红,田洪岭..基于表型性状与SNP分子标记的山西连翘种质资源遗传多样性分析[J].中国中药杂志,2026,51(15):4311-4320,10.基金项目
国家中药材产业技术体系浑源综合试验站项目(CARS-21) （CARS-21）
山西省重点研发计划项目(202402140601010) （202402140601010）
山西省中药材产业技术体系项目(2011-11) （2011-11）
山西省中药材种质资源圃项目(sxzyk202601-6) （sxzyk202601-6）
王玉庆全国老药工传承工作室项目 （）
