北京大学学报（自然科学版）2026，Vol.62Issue(4)：739-748,10.DOI:10.13209/j.0479-8023.2026.046
基于宏基因组分析灞河真核微生物群落结构及氮代谢功能
Metagenomic Analysis Revealing the Community Structure and Nitrogen Metabolic Function of Eukaryotic Microbes Along the Bahe River
摘要
Abstract
This study employed metagenomic sequencing technology and multivariate statistical methods to systematically analyze the spatial distributions and environmental driving factors of eukaryotic microbial com-munities,as well as their nitrogen metabolism genes at 15 sites along the Ba River.The results indicated sig-nificant differences in the α diversity of eukaryotic microbes among the upper,middle and lower reaches,with higher diversity in the lower reaches rather than that in the upper reaches.In terms of community composition,Bacillariophyta(48.3%)was the dominant phylum,and the relative abundance of Ciliophora remarkably increased in the middle and lower reaches.Nitrogen metabolism in eukaryotes was dominated by assimilation in the upper reaches,with a gradual increase in the abundance of transport-related genes in the middle reaches,and denitrification intensified in the lower reaches.Furthermore,TP,NO3⁻-N and NH4⁺-N significantly affected the eukaryotic microbial community structure,while NH4+-N,NO3⁻-N,Chl a,COND and salinity were the key factors driving the distribution of nitrogen metabolism genes.关键词
城市河流/真核微生物/宏基因组/氮代谢Key words
urban river/eukaryotic microbes/metagenomics/nitrogen metabolism引用本文复制引用
赵明珠,王佳文,王易初,张国华,李盈皞,杨子杰..基于宏基因组分析灞河真核微生物群落结构及氮代谢功能[J].北京大学学报（自然科学版）,2026,62(4):739-748,10.基金项目
国家自然科学基金(52209078,U2243201)、西安市青年人才托举计划(0959202513029)和中央高校基本科研业务费专项资金(xtr052025034)资助 （52209078,U2243201）
