北京林业大学学报2026，Vol.48Issue(8)：1-11,11.DOI:10.12171/j.1000-1522.20260178
栽植模式与丛枝菌根真菌对灌木根际微环境与细根形态的影响
Effects of planting patterns and arbuscular mycorrhizal fungi on rhizosphere microenvironment and fine root morphology of shrubs
摘要
Abstract
[Objective]The adaptive mechanisms underlying plant root nutrient acquisition strategies in fragile ecosystems represent a central topic in ecological research.The synergistic regulation of planting patterns and arbuscular mycorrhizal fungi(AMF)is widely recognized as a key pathway to enhance plant stress resistance and ecosystem function.However,the coupled mechanisms by which these two factors drive rhizosphere microenvironments and root morphology remain insufficiently understood.To elucidate how planting patterns and AMF synergistically shape the coupling between rhizosphere processes and root architecture,this study aims to reveal the coordinated regulatory effects of different planting patterns and AMF inoculation on rhizosphere dynamics and root system configuration.[Method]This study was conducted from 2022 to 2023 in the typical Pisha sandstone area of Jungar Banner,Ordos City,Inner Mongolia of northern China,focusing on stress-tolerant shrub species native to the region.The experiment included three planting patterns:monoculture of Clematis fruticosa and Spiraea pubescens,respectively,and a mixed-culture treatment of both shrubs combined with AMF inoculation and non-inoculation treatments.We systematically analyzed variations in rhizosphere soil microhabitats and fine root morphological traits.Two-way analysis of variance(ANOVA)was employed to test treatment effects,while partial least squares path modeling(PLS-PM)was used to dissect the driving effects of rhizosphere microenvironment on root morphology.[Result]The results demonstrated that significant interactive effects existed between planting patterns and AMF inoculation on the rhizosphere microenvironment and root morphology.Under monoculture combined with AMF inoculation,the contents of microbial biomass carbon and nitrogen in the rhizosphere of Clematis fruticosa increased significantly by 120.7%and 337.2%,respectively compared with the mixed-culture inoculation treatment(P<0.05).Meanwhile,AMF inoculation reduced nitrogen hydrolase activity under monoculture and mixed-culture treatments by 31.7%and 41.6%,respectively compared with non-inoculation controls(P<0.05).In contrast,mixed-culture enhanced soil available nitrogen content for both C.fruticosa and S.pubescens through interspecific nutrient complementarity,and synergized with AMF to decrease rhizosphere pH of the two shrubs by 0.3 and 0.1 units,respectively.Under monoculture,AMF inoculation significantly increased average root diameter of C.fruticosa and S.pubescens by 16.7%and 20.0%,respectively compared with non-inoculated controls.Without AMF inoculation,the number of root tips for both shrubs under monoculture increased significantly by 95.1%and 59.2%,respectively compared with mixed-culture treatments.Partial least squares path modeling(PLS-PM)analysis revealed that soil enzyme activity(total effect+0.894)and pH(total effect+0.548)were the core drivers of changes in root morphology.[Conclusion]By integrating multi-parameter datasets,this study demonstrates that shrub root architectural formation in the Pisha sandstone region is predicated on mycorrhizal symbiosis and mediated by remodeling of rhizosphere enzyme activity and pH.This plasticity reflects a differentiated adaptive strategy to planting patterns and inoculation treatments,offering a scientific foundation for optimizing plant-microbe resource allocation in vegetation restoration.关键词
砒砂岩区/栽植模式/丛枝菌根真菌/根际微环境/根系形态Key words
Pisha sandstone area/planting patterns/arbuscular mycorrhizal fungi/rhizosphere microenvironment/root morphology分类
农业科技引用本文复制引用
郝龙飞,孙文娜,刘婷岩,张盛晰,吴航,赵凯悦,青春..栽植模式与丛枝菌根真菌对灌木根际微环境与细根形态的影响[J].北京林业大学学报,2026,48(8):1-11,11.基金项目
中央引导地方科技发展计划项目(2025ZY0038),内蒙古自治区本级事业单位引进高层次人才科研支持项目(124001003),国家自然科学基金项目(32560307). （2025ZY0038）
