北京林业大学学报2026，Vol.48Issue(8)：80-95,16.DOI:10.12171/j.1000-1522.20260170
蚂蚁筑巢对纳帕海湿地面山森林土壤CO2排放的影响
Effects of ant nesting on soil CO2 emissions in the montane slope forests bordering Napahai Wetland,Yunnan Province of southwestern China
摘要
Abstract
[Objective]The processes and mechanisms by which ant nesting influences soil CO2 emission dynamics in the montane slope forests adjacent to the Napahai Wetland remain poorly understood.This study aims to elucidate the key pathways and driving mechanisms through which ant nesting regulates soil CO2 fluxes in these forests.[Method]We selected Formica manchu in the spruce-fir forests of the Napahai montane slope and established two treatments:ant nest soil and non-nest soil.Soil CO2 fluxes and bacterial community characteristics were measured using a Li-6400-09 soil respiration chamber and high-throughput sequencing during both dry and wet seasons.Correlation analysis and structural equation modeling were used to identify key drivers and regulatory pathways of soil CO2 emissions in ant nest soils.[Result](1)Ant nesting significantly promoted soil CO2 emissions(p<0.05),with clear dry-wet seasonal variations.Compared to non-nest soils,ant nest soils showed a 37.0%increase in CO2 flux during the dry season and a 22.0%increase during the wet season.(2)Ant nesting significantly increased the relative abundance of Proteobacteria(annual increase of 58.8%-96.3%)and significantly raised soil organic matter,total nitrogen,available phosphorus,available potassium,ammonium nitrogen,nitrate nitrogen,and pH(annual increase of 9.0%-635.0%).Conversely,ant nesting significantly reduced the relative abundance of Acidobacteria(annual decrease of 12.9%-29.6%)and significantly decreased the bacterial ACE and Chao1 indices,soil bulk density,and water content(annual decrease of 34.7%-42.8%).(3)During the dry season,CO2 flux in ant nest soils was significantly positively correlated with soil nutrients such as carbon and nitrogen and with the relative abundance of Proteobacteria,and significantly negatively correlated with soil bulk density and water content.During the wet season CO2 flux in ant nest soils was significantly positively correlated with soil temperature,carbon and nitrogen nutrients,and the relative abundance of Proteobacteria,and significantly negatively correlated with soil bulk density,water content,bacterial ACE and Chao1 indices,and the relative abundances of Acidobacteria and Actinobacteria.(4)Structural equation modeling revealed that the effect of ant nesting on soil CO2 flux differed significantly between dry and wet seasons.During the dry season,ant nesting mainly influenced CO2 emissions by regulating soil bulk density,nitrogen nutrients,and Proteobacteria.The relative abundance of Proteobacteria had a significant positive direct effect on CO2 emissions,whereas soil water content,temperature,and nitrogen nutrients indirectly affected emissions by influencing Proteobacteria.During the wet season,the regulatory pathway became more complex:bacterial α diversity exerted a significant negative direct effect on CO2 emissions,while dominant bacterial phyla(Proteobacteria and Acidobacteria)showed positive direct effects.Meanwhile,soil bulk density,temperature,and nitrogen and phosphorus nutrients indirectly regulated CO2 emissions by influencing the bacterial community.[Conclusion]Ant nesting significantly promotes soil CO2 emissions in the montane slope forests adjacent to Napahai Wetland,with a greater increase in the dry season(37.0%)than in the wet season(22.0%).The underlying pathways differ markedly between seasons:the dry season is dominated by a positive direct effect of Proteobacteria,whereas the wet season features positive direct effects from both Proteobacteria and Acidobacteria,accompanied by negative direct regulation by bacterial α diversity.Soil physicochemical factors primarily exert indirect effects by modulating the bacterial community.关键词
蚂蚁/土壤CO2排放/面山森林/细菌丰富度/细菌组成/土壤理化性质Key words
ants/soil CO2 emissions/mountain slope forests/bacterial richness/bacterial composition/soil physicochemical properties分类
农业科技引用本文复制引用
纪柏帆,陆梅,王明柳,黄敏超,任玉连,孙煜佳,屈婧,曹舒涵,杨宗涛..蚂蚁筑巢对纳帕海湿地面山森林土壤CO2排放的影响[J].北京林业大学学报,2026,48(8):80-95,16.基金项目
云南省农业联合重点项目(202501BD070001-024),云南省中青年学术技术带头人后备人才项目(202205AC160047),国家自然科学基金地区科学基金项目(42067011),云南省应用基础研究面上项目(202001AT070113),西南林业大学水土保持一流学科开放基金项目(SBK20240026),云南省教育厅科学研究项目(2025Y0837). （202501BD070001-024）
