动物医学进展2026，Vol.47Issue(8)：1-9,9.
猪源和猴源SLFN11蛋白的原核表达及其多克隆抗体制备
Prokaryotic Expression of Swine and Monkey SLFN11 Proteins and Preparation of Their Polyclonal Antibodies
摘要
Abstract
Schlafen 11(SLFN11)is an interferon-stimulated gene that plays important regulatory roles in tumorigenesis and viral infection.Polyclonal antibodies against swine and monkey SLFN11 proteins were generated to provide species-specific immunological tools for exploring the antiviral functions and mecha-nisms of the SLFN11 protein.The genes encoding swine and monkey SLFN11 proteins were amplified by PCR.Recombinant expression plasmids pET28a(+)-SLFN11-His-Swine and pET28a(+)-SLFN11-His-Monkey were constructed.The SLFN11 recombinant proteins were induced using a prokaryotic expression system and purified by Ni-NTA affinity chromatography.After emulsification,New Zealand White rabbits were immunized to generate rabbit polyclonal antibodies against the SLFN11 recombinant protein.Blood samples were collected from the marginal ear vein,and serum was separated.Enzyme-linked immunosor-bent assay(ELISA)was performed to detect the antibody titers against SLFN11 recombinant protein in the serum.The specificity of the polyclonal antibodies was characterized by Western blot and immunofluo-rescence assay(IFA).In this study,prokaryotic expression plasmids for SLFN11 were constructed.The swine and monkey recombinant SLFN11 proteins were obtained following induction with IPTG and purifi-cation.After immunizing New Zealand White rabbits,polyclonal antibodies were generated.ELISA analysis showed that the antibody titers reached 1∶2 500 000 and 1∶500 000,respectively.Western blot results confirmed that the generated polyclonal antibodies specifically recognized both swine and monkey SLFN11 recombinant proteins.Furthermore,IFA results indicated that the rabbit polyclonal antibodies specifically reacted with SLFN11 protein expressed from eukaryotic plasmids.In conclusion,swine and monkey SLFN11 recombinant proteins were successfully obtained using a prokaryotic expression system,and the polyclonal antibodies against these recombinant proteins were generated and demonstrated to have good specificity,providing tools for the study of the biological functions of the SLFN11 protein.关键词
SFLN11蛋白/原核表达/多克隆抗体Key words
SFLN11 protein/Prokaryotic expression/Polyclonal antibodies分类
农业科技引用本文复制引用
蔡茂,梁珺珹,张昂克,孙爱军,杜永坤,闫志伟,刘梗,王飞,王小格,尤龙琪,司煊瑛,李灏天,苑亚茹..猪源和猴源SLFN11蛋白的原核表达及其多克隆抗体制备[J].动物医学进展,2026,47(8):1-9,9.基金项目
国家自然科学基金(31702217) （31702217）
河南省重大科技专项项目(221100110600) （221100110600）
