动物医学进展2026，Vol.47Issue(8)：9-14,6.
猪流行性腹泻病毒感染IPEC-J2细胞的免疫相关基因筛选
Transcriptomic Analysis for Screening Immune-Related Genes in PEDV-Infected IPEC-J2 Cells
摘要
Abstract
The aim of this study is to investigate the changes in the genomic transcription levels of pig epi-demic diarrhea virus(PEDV)LJX strain in vitro,which will provide a reference basis for understanding the role of host immune-related factors in PEDV effection.In this study,we analyzed the transcriptomic changes in IPEC-J2 cells infected with the LJX strain of PEDV and identified immune-related genes and pathways.The optimal infection time of PEDV in IPEC-J2 cells was determined to be 24 hours using Real-Time PCR and indirect immunofluorescence techniques.Subsequently,cell samples infected for 24 hours were selected for transcriptome sequencing to identify differentially expressed genes(DEGs).The results showed that a total of 135 DEGs were identified after PEDV infection,including 77 up-regulated genes and 58 down-regulated genes.Four immune-related genes were identified through GO functional enrichment a-nalysis and KEGG pathway analysis.KEGG analysis found that TLR2 and FOS were significantly enriched in the Toll-like receptor signaling pathway,while CLEC4D and IL23A were significantly enriched in the C-type lectin receptor signaling pathway.The expression trends of DEGs were further validated by Real-Time PCR.This study identified four innate immunity-related genes(TLR2,FOS,CLEC4D,and IL23A)that play crucial roles in defending PEDV infection,providing insights into the mechanisms of host immune responses to PEDV intection.关键词
猪流行性腹泻病毒/猪肠上皮细胞-J2/转录组/免疫应答Key words
Porcine dpidemic diarrhea virus/IPEC-J2/Transcriptome/Immune response分类
农业科技引用本文复制引用
牛水珠,李绍梅,高婕棋,赵自亮,牟豪,张绍伟,杨柳..猪流行性腹泻病毒感染IPEC-J2细胞的免疫相关基因筛选[J].动物医学进展,2026,47(8):9-14,6.基金项目
国家生猪技术创新中心先导科技项目(NCTIP-XD/B19) （NCTIP-XD/B19）
四川省区域创新合作项目(2024YFHZ0110) （2024YFHZ0110）
国家动物疫病长期性观测监测项目(zx06s2302) （zx06s2302）
