动物医学进展2026，Vol.47Issue(8)：15-20,6.
牛支原体O256蛋白的原核表达及其免疫原性研究
Expression of M.bovis O256 Protein and Evaluation of Its Immune Efficacy
摘要
Abstract
Aimed to evaluate the immunogenicity of the recombinant protein O256 from Mycoplasma bovis,a prokaryotic expression plasmid carrying the O256 gene was successfully constructed and the recombinant protein was expressed.Mice were subcutaneously immunized with the protein to assess immune effects.Ser-um levels of IgG,IgG1,and IgG2a were measured by indirect ELISA using blood samples collected from the tail vein every 7 days.IFN-γ secretion was detected by ELISpot.The results showed that serum levels of IgG,IgG1,and IgG2a in the O256 immunized group were significantly higher than those in the PBS control group,indicating the induction of a humoral immune response.The IgG2a/IgG1 ratio indicated a bias to-ward a Th2-type immune response.Additionally,the ELISpot assay revealed that IFN-γ expression in splenic lymphocytes of the O256 group was significantly increased compared with the PBS group(P＜0.005),demonstrating the activation of a cellular immune response.In conclusion,the O256 recombinant protein of Mycoplasma bovis demonstrates strong immunogenicity,identifying it as a promising candidate antigen for the development of a vaccine against bovine mycoplasmosis.关键词
牛支原体/O256重组蛋白/抗体/干扰素Key words
M.bovis/O256 recombinant protein/Antibody/Interferon分类
农业科技引用本文复制引用
余小娇,李芮芮,李祥慧,梁佳音,盛金良,陈创夫,马忠臣,王勇..牛支原体O256蛋白的原核表达及其免疫原性研究[J].动物医学进展,2026,47(8):15-20,6.基金项目
石河子大学高层次人才科研启动项目(RCZK202456) （RCZK202456）
兵团重点研发计划项目(2024AB034-02) （2024AB034-02）
新疆维吾尔自治区"天池英才(青年博士)"计划项目(CZ004336) （青年博士）
