动物医学进展2026，Vol.47Issue(8)：27-33,7.
大肠埃希菌O157:H7噬菌体vB-EcoM-JYHZ01的分离鉴定及全基因组序列分析
Isolation,Identification and Whole Genome Sequence Analysis of Escherichia coli O157:H7 Phage vB-EcoM-JYHZ01
摘要
Abstract
In this study,we used the most common serotype of enterohemorrhagic Escherichia coli(E.coli)O157:H7 as the host bacteria.A bacteriophage strain,vB-EcoM-JYHZ01,was isolated and purified,follow-ing by further analysis of its biological characteristics and genetic evolution.The results showed that vB-EcoM.JYHZ01 could lyse 6 out of 24 E.coli strains,indicating a relatively narrow host spectrum.The opti-mal multiplicity of infection(MOI)for vB-EcoM-JYHZ01 was 0.1,with a short incubation period(10 min),a prolonged lysis period(90 min),and a lysis yield of 620 PFU/cell.Bacteriophage exhibited stable activity within the pH range of 3-11 and temperature range of 20-60℃.It maintained relatively stable activi-ty in a NaCl solutions with concentrations ranging from 0.5 to 3 mol/L.The phages titer decreased gradual-ly with prolonged ultraviolet lamps irradiation.Phage vB-EcoM.JYHZ01 is a double stranded linear DNA virus with a genome length of 115,939 bp and a GC content of 39.34％.Its genome contains 167 coding se-quence(CDSs),of which 78 encode functional proteins(46.71％).No genes related to antibiotic resistance,tRNA,lysogenic or virulence factors were identified.Whole-genome alignment showed that vB-EcoM-JY-HZ01 had 89％and 85％genome coverage with Salmonella bacteriophage SP33(GenBank accession num-ber:OR862218)and E.coli bacteriophage HMD-P3(GenBank accession number:PQ462667),and 97.04％and 97.58％sequence homology,respectively.Genetic evolution analysis of nucleotide sequences from con-served proteins(terminal large subunits)and no-conserved proteins(capsid proteins)revealed that vB-EcoM-JYHZ01 is closely related to members of the Tequintavirus genus.Overall,the results indicate that vB-EcoM-JYHZ01 is a virulent phage with notable characteristics such as acid and alkali resistance,high-temperature stability,and resilience in hyperosmotic solutions.Furthermore,its genome appears to be free of harmful genes,suggesting it is relatively safe for clinical applications.These findings provide a solid theo-retical basis and resource pool for further studies and potential clinical use of E.coli O157:H7 phages.关键词
大肠埃希菌O157:H7/噬菌体/分离纯化/生物学特性/基因组分析Key words
E.coli O157:H7/Phage/Separation and purification/Biological characteristics/Genome analysis分类
农业科技引用本文复制引用
景郡贤,李和平,彭代,贾超祥,覃婷,姚鑫炎,许淑慧,韩莹倩,吴华,王月影..大肠埃希菌O157:H7噬菌体vB-EcoM-JYHZ01的分离鉴定及全基因组序列分析[J].动物医学进展,2026,47(8):27-33,7.基金项目
河南省国际科技合作重点项目(241111521100) （241111521100）
