动物医学进展2026，Vol.47Issue(8)：65-73,9.
一测多评法同时测定救乳化毒汤中7种成分的含量
Simultaneous Determination of Seven Components in Jiuru Huadu Tang Using Quantitative Analysis of Multi-components by Single Marker
摘要
Abstract
The aim of this study was to establish a quantitative analysis of multi-components by single-marker method for the simultaneous determination of Chlorogenic acid,Ferulic acid,Caffeic acid,Chicoric acid,Apigenin,Kaempferol,Formononetin in Jiuru Huadu Tang.The separation was performed on a Shi-madzu Prominence LC-20A column(Agilent Eclipse Plus C18)(100 mm×4.6 mm,2.7 μm)with a gradi-ent elution of water(containing 0.1％phosphoric acid)(A)-acetonitrile(B)as the mobile phase,and the detection wavelength was 220 nm,the column temperature was 40℃,the flow rate was 1.0 mL/min,injec-tion volume of 3 μL.The relative correction factors of the other six components were calculated using chlo-rogenic acid as the internal standard,and their contents were determined.The results showed that the target components exhibited good linearity(r≥0.999 5)over the tested range,with the average spiked recoveries of 97.77％-102.16％and the RSDs of 1.65％-3.26％,The quantitative analysis of multi-components by sin-gle-marker method established in this study for the determination of Chlorogenic acid,Ferulic acid,Caffeic acid,Chicoric acid,Apigenin,Kaempferol,Formononetin in Jiuru Huadu Tang has the advantages of high accuracy and stability,and can be used for the quality control of Jiuru Huadu Tang.关键词
救乳化毒汤/化学成分/含量测定/一测多评Key words
Jiuru Huadu Tang/Chemical composition/Content determination/Quantitative analysis of multi-components by single-marker(QAMS)分类
农业科技引用本文复制引用
何昆,纪鹏,米文文,吴润民,马倩,严建鹏,华永丽,魏彦明..一测多评法同时测定救乳化毒汤中7种成分的含量[J].动物医学进展,2026,47(8):65-73,9.基金项目
国家自然科学基金项目(U21A20262) （U21A20262）
甘肃省科技重大专项(24ZDNA001) （24ZDNA001）
财政部和农业农村部国家现代农业产业技术体系项目(CARS-37) （CARS-37）
