广西医科大学学报2026，Vol.43Issue(4)：526-536,11.DOI:10.16190/j.cnki.45-1211/r.2026.04.009
肾福康胶囊抑制STAT3/CTGF通路改善大鼠肾间质纤维化的机制研究
Mechanism of rhubarb-astragalus herb pair in ameliorating renal interstitial fibrosis via inhibiting the STAT3/CTGF pathway in rats
摘要
Abstract
Objective:To explore the mechanism by which the rhubarb-astragalus herb pair(HPRA)inhibits re-nal interstitial fibrosis(RIF)in rats.Methods:Forty-eight male Sprague-Dawley(SD)rats were randomly di-vided into six groups with 8 rats per group:a sham group,a model group,a low-dose HPRA group,a medium-dose HPRA group,a high-dose HPRA group and a positive control group(losartan group).Except for the sham group,a rat model of RIF was established in all other groups by unilateral ureteral obstruction(UUO).Blood samples were collected from the abdominal aorta to detect the levels of serum creatinine(Scr)and blood urea ni-trogen(BUN).Hematoxylin-eosin(HE)staining was used to observe the renal histopathological changes.Immu-nohistochemistry(IHC)was performed to detect the protein expressions of α-smooth muscle actin(α-SMA),sig-nal transducer and activator of transcription 3(STAT3),and connective tissue growth factor(CTGF)in the rat re-nal tissues.Western blotting was applied to detect the protein expressions of vimentin,collagen typeⅠ(Col-Ⅰ)and phosphorylated STAT3(p-STAT3)in the rat renal tissues.Quantitative real-time polymerase chain reaction(RT-qPCR)was used to assess the mRNA expressions of α-SMA,Col-Ⅰ,vimentin,STAT3,and CTGF in the rat renal tissues.Double immunofluorescence staining was conducted to evaluate the co-expressions of CTGF ⁺ and STAT3⁺in the rat renal tissues.Results:Compared with the sham group,the model group exhibited dilated renal tubules and vacuolar degeneration in the rat renal tissues.Meanwhile,the levels of Scr and BUN,and the mRNA and protein expressions of α-SMA,Col-Ⅰ,vimentin,STAT3 and CTGF,the protein expression level of p-STAT3,as well as the co-expression proportion of CTGF ⁺ and STAT3 ⁺ were significantly increased in the model group(P＜0.05).All HPRA dose groups showed markedly alleviated renal pathological injury compared with the model group(P＜0.05).Meanwhile,the levels of Scr and BUN,the mRNA and protein expressions of α-SMA,vimen-tin,STAT3 and CTGF,as well as the proportion of CTGF⁺ and STAT3⁺ co-expressions were significantly decreas-ed(P＜0.05).Conclusion:HPRA can significantly inhibit the progression of RIF in rats,which may be related to its inhibition of the STAT3/CTGF signaling pathway in renal tissue.关键词
肾福康胶囊/慢性肾脏病/肾间质纤维化/输尿管梗阻/STAT3/CTGF信号通路/α-平滑肌肌动蛋白/细胞外基质/氯沙坦Key words
rhubarb-astragalus herb pair/chronic kidney disease/renal interstitial fibrosis/ureteral obstruction/STAT3/CTGF signaling pathway/α-smooth muscle actin/extracellular matrix/losartan分类
医药卫生引用本文复制引用
梁志伟,冯祥乾,邹小琴,杨玉芳,杜炜,李秀兰,钟小斌,李思萍..肾福康胶囊抑制STAT3/CTGF通路改善大鼠肾间质纤维化的机制研究[J].广西医科大学学报,2026,43(4):526-536,11.基金项目
国家自然科学基金资助项目(82360811) （82360811）
广西自然科学基金资助项目(2023GXNSFAA026233) （2023GXNSFAA026233）
