贵州农业科学2026，Vol.54Issue(8)：14-21,8.DOI:10.3969/j.issn.1001-3601.2026.08.002
喷施谷氨酸对苦荞麦幼苗生长及相关基因表达的影响
Effects of Spraying Glutamic Acid on Seedling Growth and Expression of Related Genes in Tartary Buckwheat
摘要
Abstract
[Objective]The effects of glutamic acid on growth and related gene expression of tartary buckwheat seedlings were explored to provide a theoretical basis for increasing crop biomass by using exogenous substances.[Method]The related growth indexes of tartary buckwheat seedlings of Chuanqiao No.1 sprayed with different concentrations(10 μmol/L,20 μmol/L,30 μmol/L,40 μmol/L,50 μmol/L and 60 μmol/L)of glutamic acid were determined.The expression level of the genes related to chlorophyll synthesis and N transport was analyzed to investigate the effects of spraying glutamic acid on growth of tartary buckwheat seedlings.[Result]The height,fresh weight,SPAD value and N content of tartary buckwheat seedlings sprayed with 40 µmol/L glutamic acid increased by 8%,28%,52%and 35%compared with CK(spraying clean water)respectively,which indicated that spraying glutamic acid promoted growth of tartary buckwheat seedlings.Spraying 40 µmol/L glutamic acid obviously promoted expression of FtDCL and FtGLK1 genes related to chlorophyll synthesis and FtAMT1-1 and FtNRT2.3 genes related to N transport in tartary buckwheat.The maximum expression level of FtDCL and FtGLK1 genes at 12 h increased by 7.63 times and 8.01 times compared with CK,respectively.The maximum expression level of FtAMT1-1 and FtNRT2.3 genes at 12 h and 24 h increased by 8.33 times and 6.21 times compared with CK,respectively.[Conclusion]Spraying 40 µmol/L glutamic acid not only increases height,fresh weight,and chlorophyll and N content of tartary buckwheat seedlings but also promotes expression of genes related to chlorophyll synthesis and N transport of tartary buckwheat seedlings.关键词
苦荞麦/谷氨酸/幼苗生长/叶绿素/N含量/生理生化/基因表达Key words
tartary buckwheat/glutamic acid/seedling growth/chlorophyll/N content/physiology and biochemistry/gene expression分类
农业科技引用本文复制引用
吴香琴,张萌,杨洪兵..喷施谷氨酸对苦荞麦幼苗生长及相关基因表达的影响[J].贵州农业科学,2026,54(8):14-21,8.基金项目
国家自然科学基金项目(31371552) （31371552）
