贵州农业科学2026，Vol.54Issue(8)：33-41,9.DOI:10.3969/j.issn.1001-3601.2026.08.004
CRISPR/Cas9编辑DTH8基因改良优质稻大粒香抽穗期
Editing DTH8 Gene by Using CRISPR/Cas9 Technology to Improve the Heading Date of Dalixiang,a High-quality Rice Variety
摘要
Abstract
[Objective]The DTH8 gene in rice was modified by using CRISPR/Cas9 gene editing technology to shorten the growth period of Dalixiang(a high-quality rice variety)without influencing its excellent agronomic traits and provide a new strategy for improvement of rice varieties.[Method]Taking the high-quality rice variety Dalixiang from Guizhou as the material,specific sgRNAs targeting functional domains of DTH8 were designed by using CRISPR/Cas9 gene editing technology and introduced into Dalixiang calli via agrobacterium-mediated transformation.The mutant rice plants were sequenced and identified after PCR screening.The exogenous genes of the positive rice plants were eliminated and screened to obtain the transgene-free homozygous lines.The agronomic traits of heading date,thousand-grain weight,grain shape,milled rice rate,chalkiness rate,yield per plant and seed-setting percentage of wild and mutant rice plants were determined.[Result]Four independent T0 positive transgenic lines without exogenous genes were successfully obtained and bred.Two transgene-free homozygous mutants(J1186-2-2 and J1186-25-1)were isolated by PCR amplification and sequencing.The heading date of J1186-2-2 and J1186-25-1 was 6 d and 5 d earlier than the wild type respectively and their rice quality was no significant variation.The yield and seed-setting percentage of J1186-25-1 increased by 5.55%and 3.63%,but the yield and seed-setting percentage of J1186-2-2 decreased by 8.77%and 8.56%.[Conclusion]The CRISPR/Cas9-edited DTH8 gene can effectively shorten the heading date of mutant plant J1186-2 and J1186-25-1 of Dalixiang with a stable rice quality.关键词
水稻/大粒香/DTH8/CRISPR/Cas9/基因编辑/抽穗期/生育期Key words
rice/Dalixiang/DTH8/CRISPR/Cas9/gene editing/heading stage/growth stage分类
农业科技引用本文复制引用
吴娴,王倩,宫彦龙,李文扣,龙武华,朱速松,刘雪薇..CRISPR/Cas9编辑DTH8基因改良优质稻大粒香抽穗期[J].贵州农业科学,2026,54(8):33-41,9.基金项目
贵州省科技计划项目(黔科合基础-ZK[2024]一般551) （黔科合基础-ZK[2024]一般551）
贵州省育种科研基础平台创新能力建设(黔科合服企[2022]014) （黔科合服企[2022]014）
喀斯特山区特色作物生物育种创新能力建设(黔科合服企[2024]003-1) （黔科合服企[2024]003-1）
喀斯特山区特色粮油作物生物育种部省共建重点实验室科研能力条件建设(黔科合服企[2024]003-2) （黔科合服企[2024]003-2）
