贵州农业科学2026，Vol.54Issue(8)：84-94,11.DOI:10.3969/j.issn.1001-3601.2026.08.010
不同作物轮作下的植烟土壤微生物群落结构和生态功能
Microbial Community Structure and Ecological Function in Tobacco-planting Soil Under Different Crop Rotations
摘要
Abstract
[Objective]The effects of different crop rotations on the microbial community structure and ecological function of tobacco-growing soil were investigated,which provided theoretical basis for the green prevention and control strategy of tobacco soil-borne diseases based on microbial regulation.[Method]Illumina NovaSeq high-throughput sequencing technology was used to analyze the composition,network structure and microbial function of soil fungal and bacterial communities in the rotation of Longya cabbage,Vicia villosa and pea with tobacco.[Result]Microbial community structure analysis showed that compared with winter fallow,all three types of subsequent crops reduced the α-diversity of soil fungal and bacterial communities,and there were significant differences in β-diversity as well;The relative abundance of Mortierella,Penicillium and Fusarium were increased,while the relative abundance of Plectosphaerella was decreased.The relative abundance of Proteobacteria was increased as that of Acidobacteriota was decreased;Mortierella and Penicillium were mainly involved in the establishment of fungal correlation network in soil,while Chujaibacter and Acidothermus were mainly involved in the establishment of bacterial correlation network.Ecological function analysis showed that the three types of aftercrop could significantly increase the abundance of soil saprotrophic fungi and significantly reduce the abundance of pathotrophic fungi,but there was no significant difference in the function and phenotype of bacterial communities.[Conclusion]The three kinds of subsequent crops,including Longya cabbage,V.villosa and pea,can reduce the microbial diversity and the relative abundance of pathotrophic fungi in tobacco-growing soil,and can increase the relative abundance of beneficial microorganisms.关键词
烟草/微生物/群落结构/生态功能/多样性/高通量测序Key words
tobacco/microorganism/community structure/ecological function/diversity/high-throughput sequen-cing分类
农业科技引用本文复制引用
张海,赵玳琳,何海永,董延鑫,王莹,张权,谭清群,李继业..不同作物轮作下的植烟土壤微生物群落结构和生态功能[J].贵州农业科学,2026,54(8):84-94,11.基金项目
贵州省烟草公司安顺市公司科技项目(2023ASXM01号) （2023ASXM01号）
安顺市烟草病毒病绿色防控技术集成及推广应用(2023XM34) （2023XM34）
贵州省科技计划项目农业入侵物种绿色防控创新能力建设(黔科合服企[2023]011) （黔科合服企[2023]011）
