黑龙江八一农垦大学学报2026，Vol.38Issue(4)：40-49,10.DOI:10.3969/j.issn.1002-2090.2026.04.006
猪流行性腹泻病毒截短S蛋白纳米颗粒的制备及其对小鼠免疫效果评价
Preparation of Nanoparticles Displaying Truncated S Protein of Porcine Epidemic Diarrhea Virus and Evaluation of Their Immunogenicity in Mice
摘要
Abstract
Emergence of variant strains of Porcine Epidemic Diarrhea Virus(PEDV)compromises the protective efficacy of conventional vaccines,which urgently requires novel rapid and efficient vaccine development strategies.In this study,a ferritin nanoparticle-based antigen display platform was constructed to present S10,a key epitope derived from the S1 protein of variant PEDV.After codon optimization,the S10 gene fused with a C-terminal VL12.3 tag was cloned into the pET-32a vector,followed by prokaryotic expression in Escherichia coli and purification to obtain recombinant S10-VL12.3 protein.Meanwhile,Helicobacter pylori-derived ferritin(Fer)with an N-terminal HTT tag was expressed and purified.Relying on specific covalent interaction between HTT and VL12.3,the two purified proteins were assembled in vitro into PEDV S10-Fer conjugates.Transmission electron microscopy revealed that the assembled S10-Fer existed as uniform spherical nanoparticles with characteristic surface protrusions and an average diameter of 32.7 nm.Female BALB/c mice were immunized with prepared formulations,and serum samples were collected two weeks after the third immunization.Virus neutralization assay demonstrated that S10-Fer nanoparticles elicited markedly superior immune responses compared with soluble S10 protein;the neutralizing antibody titer in the S10-Fer group was extremely significantly higher than that of the S10 group(P＜0.01).In conclusion,the ferritin nanoparticle scaffold effectively improves antigen immunogenicity and triggers robust immune responses in mice,providing a promising strategy for rapid development of nanoparticle vaccines against variant PEDV and other pathogenic viruses.关键词
猪流行性腹泻病毒变异株/S1 蛋白表位/铁蛋白纳米颗粒/小鼠/免疫原性Key words
variant porcine epidemic diarrhea virus/S1 protein epitope/ferritin nanoparticle/mice/immunogenicity分类
农业科技引用本文复制引用
杨丹,张美辰,曹莹莹,姜卫岳,孙东波..猪流行性腹泻病毒截短S蛋白纳米颗粒的制备及其对小鼠免疫效果评价[J].黑龙江八一农垦大学学报,2026,38(4):40-49,10.基金项目
国家自然科学基金项目(32503035) （32503035）
新时代龙江优秀硕士、博士学位论文项目(LJYXL2024-043) （LJYXL2024-043）
大庆市指导性科技计划项目(zd-2025-035) （zd-2025-035）
黑龙江八一农垦大学定向培养科研启动基金项目(XYB202508). （XYB202508）
