华南农业大学学报2026，Vol.47Issue(5)：867-877,11.DOI:10.7671/j.issn.1001-411X.202508013
花生粕酶解工艺优化及产物对马铃薯生长和土壤质量的影响
Peanut meal enzymatic hydrolysis process optimization and effects of its products on potato growth and soil quality
摘要
Abstract
[Objective]To optimize the enzymatic hydrolysis process of peanut meal,and investigate the effects of the resulting hydrolysate as a partial substitute for inorganic N fertilizer on potato growth and soil quality,aiming to promote the resource utilization of peanut meal waste and the sustainable development of potato production.[Method]A four-factor,three-level Box-Behnken design experimental design was used to optimize reaction time,initial reaction pH,reaction temperature,and enzyme addition amount for preparing an enzymatic hydrolysate(M)with high protein hydrolysis degree.Under field conditions,the effects of enzymatic hydrolysate and traditionally fermented peanut meal broth(F)on potato growth and soil quality were compared at inorganic N fertilizer substitution rates of 5%(M5,F5),10%(M10,F10),15%(M15,F15),and 20%(M20,F20).[Result]The optimal conditions for peanut meal protein hydrolysis were:Reaction time of 4.5 h,initial reaction pH of 8.6,reaction temperature of 54.9℃,and enzyme addition amount of 900.8 U·g-1,with an average hydrolysis degree of 25.02%.After optimization,the nutrient contents of enzymatic hydrolysate were:Total N 5.10 g·L-1,total P 5.30 g·L-1,total K 9.70 g·L-1,and organic matter 50.40 g·L-1.The M10 treatment exhibited the optimal effect on increasing the total potato yield and large potato production,with the total yield and large potato yield significantly increasing by 26.63%and 30.90%,respectively;Tuber N,P,K,and dry matter accumulated amounts significantly increased by 19.04%,22.47%,29.32%,and 31.86%higher than those of the control,respectively.For the M20 treatment,soil pH significantly increased by 5.70%,compared with the control,while organic matter content and electrical conductivity significantly increased by 13.48%and 40.06%,respectively.The alkaline-hydrolyzable N content reached a peak of 106.26 mg·kg-1 in the M15 treatment,which was 5.70%significantly higher than that of the control.The α diversity indices of the bacterial community in the M10 treatment were obviously higher than those in the other treatments.The relative abundances of the plant-beneficial bacteria Terrabacter,Sphingomonas and Arthrobacter in M5 treatment were significantly increased compared with F5 treatment.[Conclusion]After process optimization,both the hydrolysis degree and nutrient content of peanut meal are improved.Replacement of partial inorganic N fertilizer with the enzymatic hydrolysate can be regarded as an effective measure for potato green production,but its long-term potential and large-scale promotion still need to be verified.关键词
花生粕/响应面优化/酶解/无机N肥替代/马铃薯/土壤环境Key words
Peanut meal/Response surface optimization/Enzymatic digestion/Inorganic N fertilizer replacement/Potato/Soil environment分类
农业科技引用本文复制引用
丘智晃,黄吴芷卉,张心怡,何丹艺,冯紫荟,温斌海,黄可欣,邓兰生..花生粕酶解工艺优化及产物对马铃薯生长和土壤质量的影响[J].华南农业大学学报,2026,47(5):867-877,11.基金项目
国家自然科学基金(423772211) （423772211）
国家重点研发计划(2023YFD2300805) （2023YFD2300805）
