河南农业科学2026，Vol.55Issue(8)：91-99,9.DOI:10.15933/j.cnki.1004-3268.2026.08.010
羧酸酯酶基因BoαE1介导的韭菜迟眼蕈蚊对辛硫磷的解毒机制
The Carboxylesterase Gene BoαE1 Mediated Detoxification Mechanism of Phoxim in Bradysia odoriphaga
摘要
Abstract
Bradysia odoriphaga is a major pest of Chinese chive and has developed resistance to the commonly used organophosphorus insecticide phoxim.To elucidate the detoxification mechanism of B.odoriphaga against phoxim,a combination of methods including carboxylesterase-specific inhibitor synergism assay,quantitative real-time PCR,RNA interference(RNAi),and in vitro inhibition assay were employed.The results showed that under exposure to the 30%lethal concentration(LC30)of phoxim,carboxylesterase activity in third-instar larvae increased significantly.The carboxylesterase activities at 12,24,36,and 48 h post-treatment were 40.03,32.91,30.03,and 27.98 μmol/(mg·min),representing 2.44-,1.68-,1.58-,and 1.82-fold increases compared to the blank control.Following treatment with the specific carboxylesterase inhibitor triphenyl phosphate(TPP),the mortality of the larvae reached 41.67%,which was 8.34 percentage points higher(a relative increase of 25.0%)than the control group not fed with TPP.Under phoxim stress,the expression level of the carboxylesterase gene BoαE1 was significantly up-regulated.Knockdown of BoαE1 by RNAi resulted in a corrected mortality rate of 56.25%,representing a 30.44 percentage point increase(a relative increase of 117.9%)compared to the control larvae without gene silencing.In vitro inhibition assays further demonstrated that phoxim inhibited the activity of the recombinant BoαE1 protein with a half-maximal inhibitory concentration(IC50)of 4.020×10-4 mg/L.In conclusion,the carboxylesterase gene BoαE1 is involved in the detoxification metabolism of phoxim in B.odoriphaga and plays a crucial role in the development of resistance to organophosphorus insecticides.关键词
韭菜迟眼蕈蚊/辛硫磷/羧酸酯酶/RNA干扰/解毒代谢Key words
Bradysia odoriphaga/Phoxim/Carboxylesterase/RNAi/Detoxification metabolism分类
农业科技引用本文复制引用
解园,张晓晨,肖宏丽,李耀发,刘颖超,唐博文..羧酸酯酶基因BoαE1介导的韭菜迟眼蕈蚊对辛硫磷的解毒机制[J].河南农业科学,2026,55(8):91-99,9.基金项目
国家自然科学基金项目(32202299) （32202299）
国家现代农业产业技术体系项目(CARS-21) （CARS-21）
河北省省级科技计划项目(236Z6504G) （236Z6504G）
河北省高等学校科学技术研究项目(BJK2024007) （BJK2024007）
河北省现代农业产业技术体系项目(HBCT2024110207) （HBCT2024110207）
保定市科技计划项目(2472P007) （2472P007）
河北农业大学引进人才启动科研基金项目(YJ2020012) （YJ2020012）
