林产化学与工业2026，Vol.46Issue(4)：17-26,10.DOI:10.20195/j.issn.0253-2417.2025043
福建柏叶总黄酮提取工艺优化及抑菌活性研究
Optimization of Extraction Technology and Antifungal Activities of Total Flavonoids Extract From the Leaves of Fokienia hodginsii
摘要
Abstract
Using the leaves of Fokienia hodginsii as the research material,the ultrasound-assisted extraction process of total flavonoids was optimized through single-factor experiments and orthogonal test combined with response surface methodology.The content of flavonoids in the extract was determined by ultraviolet spectrophotometry,and its antifungal activity was evaluated.The results showed that the optimal extraction conditions for total flavonoids from Fokienia hodginsii leaves were as follows:ethanol volume fraction of 65％,liquid-to-solid ratio of 20:1(mL:g),extraction time of 61 min,ultrasound power of 460 W,and extraction temperature of 75℃.Under these conditions,the theoretical extraction yield of total flavonoids reached22.15％.The verification experiments showed an average extraction yield of 22.29％(RSD=3.52％),and the scale-up experiments yielded an average extraction rate of 22.32％(RSD=3.76％),indicating excellent stability of the process.At a mass concentration of 2 g/L,the total flavonoids had the inhibition rates of 66.77％and 61.25％against Sclerotium rolfsii and Colletotrichum gloeosporioides,with the half maximal effective concentrations(EC50)of 0.89 and1.10 g/L,respectively.The total flavonoids inhibited mycelial growth by reducing the activities of three fungal protective enzymes,namely catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD),which impaired the self-detoxification capacity of the fungal cells.Meanwhile,they induced membrane lipid peroxidation and disrupted the energy metabolism process required for mycelial growth,thereby suppressing the proliferation of plant pathogenic fungi.关键词
黄酮/响应面法/工艺优化/抑菌活性Key words
flavone/response surface method/process optimization/antifungal activity分类
化学化工引用本文复制引用
付硕硕,徐孟飞,龚伟勇,李渊顺,徐会有,倪林..福建柏叶总黄酮提取工艺优化及抑菌活性研究[J].林产化学与工业,2026,46(4):17-26,10.基金项目
福建省林业科技项目(2023FKJ11) （2023FKJ11）
福建农林大学科技创新专项基金项目(KFb22019XA) （KFb22019XA）
