广东石油化工学院学报2026,Vol.36Issue(3):26-31,6.DOI:10.26962/j.cnki.1991.2026.0046
木质纤维素降解菌的筛选、鉴定及酶学性质
Screening and Identification of Lignocellulose Degrading Bacteria and Study in Its Enzymatic Properties
摘要
Abstract
To provide experimental data and theoretical guidance for the high-value utilization of lignocellulose,strains with high decomposition efficiency for lignocellulose were screened and their enzymatic properties were studied.In this study,a strain of Bacillus subtilis was screened from Guangdong Maoming Lutiankuang Ecology Park.The xylanase and endoglucanase produced by the strain had optimum temperatures of 50℃and 60℃,and optimum pH of 7.0 and 6.0,respectively.while xylanase maintained good pH stability under medium-alkaline conditions.Metal ions and chemical reagents have varying degrees of inhibitory effects on xylanase,while Cu2+and SDS have a slight promoting effect on endoglucanase.The excellent performance of lignocellulose degrading enzymes secreted by Bacillus altitudinis strain OU9 provides enormous potential value for the efficient green degradation and exploitation as well as application of lignocellulose.关键词
木质纤维素/菌株筛选/木聚糖酶/内切葡聚糖酶/酶学性质Key words
lignocellulose/strain screening/xylanase/endoglucanase/enzymatic properties分类
资源环境引用本文复制引用
区晓阳,廖育祥,李莉梅,陆光远..木质纤维素降解菌的筛选、鉴定及酶学性质[J].广东石油化工学院学报,2026,36(3):26-31,6.基金项目
广东石油化工学院人才引进项目(2020rc046) (2020rc046)
广东省乡村振兴科技专项(2022DZXHT072,2023S018087,2023S002021) (2022DZXHT072,2023S018087,2023S002021)