广东石油化工学院学报2026，Vol.36Issue(3)：37-41,5.DOI:10.26962/j.cnki.1991.2026.0048
1株港口码头石油降解菌的分离与鉴定
Isolation and Identification of a Strain of Oil-Degrading Bacteria in Port
摘要
Abstract
Oil pollution has a profound effect on the ecology along the port.It is an important direction for oil pollution remediation to study and excavate microorganisms capable of oil degradation.Through enrichment culture,oil plate screening and blood plate identification,a strain of oil-degrading bacteria producing surfactant was screened from the dock coast.Based on 16S rRNA gene sequencing analysis,the bacterium was identified as Microbacterium esteraromaticum and named M.esteraromaticum BS-1.The fermentation solution of the strain BS-1 could reduce the surface tension of water from 57.1 mN/m to 28.7 mN/m,and the degradation rate of crude oil reached 31.6%.The surfactants produced by BS-1 growth and metabolism were identified as glycolipids containing aldose groups by TLC thin layer chromatography,characteristic color development and infrared spectroscopy.The results can provide reference for developing new biosurfactants and further studying oil pollution bioremediation technology.关键词
生物表面活性剂/酯香微杆菌/石油降解Key words
biosurfactant/Microbacterium esteraromaticum/petroleum degradation分类
资源环境引用本文复制引用
刘金丰,萧允艺,刘杰凤..1株港口码头石油降解菌的分离与鉴定[J].广东石油化工学院学报,2026,36(3):37-41,5.基金项目
广东省普通高校重点领域专项(乡村振兴)(2020ZDZX1056) （乡村振兴）
广东省普通高校特色创新项目(自然科学)(2023KTSC.X090) （自然科学）
茂名市科技计划项目(2024059) （2024059）
