热带作物学报2026，Vol.47Issue(8)：1908-1915,8.DOI:10.3969/j.issn.1000-2561.2026.08.002
木薯转录因子MeMYBS1的克隆与表达分析
Cloning and Expression Analysis of Transcription Factor MeMYBS1 in Cassava
摘要
Abstract
MYB transcription factors play crucial roles in plant growth and development,secondary metabolism,and responses to environmental stresses.In this study,a 1R-MYB transcription factor gene,MeMYBS1,was cloned from cassava(Manihot esculenta Crantz)cultivar South China 8(SC8)using RT-PCR technology.Bioinformatics analysis using online tools such as ExPasy and NCBI-CDD revealed that the full-length of MeMYBS1 was 1008 bp,encoding a protein of 335 amino acids with a predicted molecular weight of 36.3 kDa and an isoelectric point of 6.46.Subcellular localization analysis via PEG-mediated transient expression in cassava protoplasts demonstrated that MeMYBS1 was localized in the nucleus.qRT-PCR tissue expression analysis showed that MeMYBS1 exhibited high expression abun-dance in cassava storage roots.Furthermore,during the process of post-harvest physiological deterioration(PPD)in South China 9(SC9)storage roots,the expression level of MeMYBS1 showed a significant downward trend as storage time extended.The results preliminarily characterized the expression patterns of MeMYBS1 during cassava PPD,pro-viding a theoretical foundation for further elucidating the molecular mechanisms by which this gene regulates PPD in cassava.关键词
木薯/1R-MYB转录因子/生物信息学分析/组织表达模式/PPDKey words
cassava/1R-MYB transcription factor/bioinformatics analysis/tissue expression pattern/postharvest physiological deterioration(PPD)分类
农业科技引用本文复制引用
伍家海,刘沁雲,蔡杰,安飞飞,罗秀芹..木薯转录因子MeMYBS1的克隆与表达分析[J].热带作物学报,2026,47(8):1908-1915,8.基金项目
国家重点研发项目(No.2023YFD1200200) （No.2023YFD1200200）
海南省重点研发三亚崖州湾科技城科技创新联合项目(No.ZDYF2025GXJS135) （No.ZDYF2025GXJS135）
热带作物生物育种全国重点实验室科研项目(No.NKLTCBCXTD30). （No.NKLTCBCXTD30）
