热带作物学报2026，Vol.47Issue(8)：1949-1958,10.DOI:10.3969/j.issn.1000-2561.2026.08.006
秋石斛不同外植体对MSO的敏感性及发根农杆菌转化效率比较
Comparison of Sensitivity to MSO and Transformation Efficiency of Agrobacterium rhizogenes among Different Explants of Phalaenopsis-type Dendrobium Hybrids
摘要
Abstract
Phalaenopsis-type Dendrobium hybrids(Den-Phals)are important tropical ornamental plants,and establish-ing an efficient and stable genetic transformation system is crucial for gene functional research and molecular breeding.This study aimed to optimize selection conditions for genetic transformation and evaluate the transformation efficiency of different Agrobacterium rhizogenes strains.Using D.Noble Woman and D.Sonia Hiasakul as materials,the effects of different concentrations of L-methionine sulfoximine(MSO)on the growth of embryogenic calli(ECs),protocorms,calli and protocorm-like bodies(PLBs)were tested to determine optimal selection concentrations.The transformation efficiencies of three A.rhizogenes strains(K599,ATCC 15834,and MSU440)on four explant types of both cultivars were compared and verified through GUS staining and bar gene PCR detection.Results showed significant differences in MSO sensitivity among different explants.The complete lethal concentration for Noble Woman ECs and protocorms was 20 μmol/L and 15 μmol/L,respectively,and that for Sonia Hiasakul PLBs and callus tissues was 8 μmol/L and 10 μmol/L,respectively.The optimized selection pressure effectively reduced false positive rates.Regarding transfor-mation efficiency,strains ATCC15834 and MSU440 showed significantly higher transformation efficiency than K599 on most explants,with ATCC15834 achieving 7.45%positive rate for Noble Woman ECs and MSU440 reaching 8.42%for Sonia Hiasakul callus tissues.This study clarified applicable MSO concentrations for different explants of two Den-Phals and identified ATCC15834 and MSU440 as suitable A.rhizogenes strains for Den-Phals transformation,pro-viding technical references for further optimization of genetic transformation systems and molecular breeding applica-tions.关键词
秋石斛/遗传转化/MSO/筛选剂/发根农杆菌/转化效率Key words
Phalaenopsis-type Dendrobium hybrids/genetic transformation/MSO/screening agent/Agrobacterium rhi-zogenes/transformation efficiency分类
农业科技引用本文复制引用
王凯乐,李亚梅,郑碧兰,李崇晖..秋石斛不同外植体对MSO的敏感性及发根农杆菌转化效率比较[J].热带作物学报,2026,47(8):1949-1958,10.基金项目
中央级公益性科研院所基本科研业务费专项(No.1630032022002) （No.1630032022002）
国家自然科学基金项目(No.32502759). （No.32502759）
