热带作物学报2026，Vol.47Issue(8)：1990-1999,10.DOI:10.3969/j.issn.1000-2561.2026.08.010
产期调节龙眼成花过程中顶芽和叶片的H2O2、NO含量分布变化
Effects of Off-season Longan on H2O2 and NO Content and Distribu-tion in Terminal Buds and Leaves during Flowering
摘要
Abstract
Potassium chlorate is a key agent for inducing off-season flowering in longan(Dimocarpus longan Lour.),and its strong oxidizing property can cause oxidative stress in trees.Reactive oxygen species(e.g.,H2O2)and reactive ni-trogen species(e.g.,NO),as key signaling molecules of oxidative stress,and their distribution and accumulation in ter-minal buds and leaves may regulate the flower bud differentiation process of off-season longan.In this study,9-year-old'Chuliang'longan trees were used as materials.Potassium chlorate was applied to the soil for flower induction,com-bined with foliar spraying of H2O2 generator(methyl viologen,MV),H2O2 scavenger(dimethylthiourea,DMTU),NO generator(sodium nitroprusside,SNP)and NO synthase inhibitor(L-NNA).The content changes and tissue distribution characteristics of H2O2 and NO in terminal buds and leaves during flower bud differentiation were systematically ana-lyzed.The MV and SNP treatments significantly advanced the flower bud differentiation process of off-season longan,and the pure flower bud rate was significantly higher than that of the control at 28 days after treatment.DMTU and L-NNA treatments inhibited or slowed down the flowering process.H2O2 was mainly distributed in the apical meristem and rudimentary leaves of terminal buds,and first accumulated in the main veins of leaves and then diffused to the mesophyll.The peak of H2O2 in leaves appeared earlier than that in terminal buds.SNP and MV treatments could en-hance the fluorescence intensity of H2O2,while DMTU treatment significantly scavenged H2O2.The NO content reached a peak at 7 days after flower induction,SNP treatment could significantly increase the NO content in terminal buds and leaves,and L-NNA treatment had no significant effect on NO accumulation but inhibited flowering.In conclusion,H2O2 and NO,key signaling molecules,jointly promote the flowering of off-season longan.The content changes and tissue distribution characteristics would provide a theoretical basis for analyzing the oxidative stress mechanism of potassium chlorate-induced longan flower bud differentiation.关键词
龙眼/产期调节/成花/H2O2/NOKey words
longan(Dimocarpus longan Lour.)/off-season/flowering/H2O2/NO分类
农业科技引用本文复制引用
杨子琴,王家保,张蕾,洪继旺,李松刚..产期调节龙眼成花过程中顶芽和叶片的H2O2、NO含量分布变化[J].热带作物学报,2026,47(8):1990-1999,10.基金项目
国家自然科学基金面上项目(No.32472693) （No.32472693）
海南省现代农业产业技术体系建设专项(No.HNARS-08). （No.HNARS-08）
