日用化学品科学2026，Vol.49Issue(8)：36-41,6.DOI:10.3969/j.issn.1006-7264.2026.08.006
基于双光子显微镜的光子嫩肤术后皮肤评估方法
Two-photon microscopy-based assessment of skin changes following photo-rejuvenation treatment
司小童 1侯盼 1王俊杰 2孙蕾3
作者信息
- 1. 通标标准技术服务(青岛)有限公司,山东 青岛 266000
- 2. 北京超维景生物科技有限公司,北京 100000
- 3. 通标标准技术服务(上海)有限公司,上海 200233
- 折叠
摘要
Abstract
This study enrolled 24 healthy adult female volunteers who underwent intense pulsed light(IPL)photo-rejuvenation treatment and were evaluated before and after the procedure using instrumental skin assessments.Among them,five participants underwent non-invasive in vivo imaging by two-photon microscopy.After IPL treatment,the skin barrier showed a transient disruption followed by progressive recovery and remodeling.Improvements in wrinkles at the outer canthus and nasolabial folds were observed from day 7 after treatment and persisted throughout the study period.Two-photon microscopy revealed enhanced stratum corneum organization and increased epidermal thickness.At the dermal-epidermal junction,greater structural undulation was observed,accompanied by increased collagen density and more organized elastic fiber architecture.Quantitative image analysis demonstrated an increase in the second harmonic generation-to-autofluorescence aging index of dermis(SAAID)and a decrease in the elastin-to-collagen optical ratio(ELCOR),indicating dermal remodeling and attenuation of photoaging-related characteristics.These findings suggest that two-photon microscopy enables objective,non-invasive,molecular-level assessment of IPL-induced barrier repair and dermal remodeling,providing mechanistic insights that complement conventional skin instrumental evaluations.关键词
表皮层/真皮层/强脉冲光/光子嫩肤Key words
epidermis/dermis/intense pulsed light/photo-rejuvenation分类
化学化工引用本文复制引用
司小童,侯盼,王俊杰,孙蕾..基于双光子显微镜的光子嫩肤术后皮肤评估方法[J].日用化学品科学,2026,49(8):36-41,6.
