水产学报2026，Vol.50Issue(9)：87-100,14.DOI:10.11964/jfc.20260415517
尖孢镰孢菌侵染对红鱼干风味品质及双向代谢特征的影响
Effects of Fusarium oxysporum infection on flavor quality and bidirectional metabolic characteristics of dried Lutjanus erythropterus fillets
摘要
Abstract
Dried Lutjanus erythropterus fillets are susceptible to fungal contamination during storage,resulting in flavor deteri-oration and food safety risks.Fusarium oxysporum has been identified as one of the predominant spoilage fungi in dried L.erythropterus fillets,but its spoilage mechanism remains unclear.This study investigated the effects of F.oxysporum infection on the flavor quality,food safety,and bidirectional metabolic characteristics of dried L.erythropterus fillets.A 40-day storage experiment was conducted using a non-inoculated control group(CK)and an F.oxysporum-inoculated group(FO).Dynamic changes in fungal colony counts,deoxynivalenol(DON),moisture content,and adenosine monophosphate(AMP)were mon-itored.Free amino acids,volatile compounds,and untargeted metabolomics were analyzed to characterize flavor and metabolic alterations.The fungal population in the FO group increased rapidly and reached a maximum of 260 CFU/g on day 24.DON concentration remained low before day 20 but increased sharply thereafter and stabilized at 12.93-14.47 μg/kg.Moisture con-tent and AMP in the FO group exhibited a three-stage variation pattern of decline,increase,and subsequent decline.Compared with the CK group,umami,sweet,and bitter amino acids increased significantly and reached their highest levels on day 40,with excessive accumulation of bitter amino acids contributing to taste deterioration.F.oxysporum infection reduced desirable volatile compounds,including aldehydes and esters,while promoting the accumulation of spoilage-related amines and pyrazines.Phenylacetaldehyde was identified as a characteristic biomarker,and days 12-20 represented the critical period for flavor deterioration.Untargeted metabolomics revealed significant temporal metabolic differentiation,with 198 metabolites upregulated and 131 downregulated on day 32 compared with day 0.Differential metabolites were mainly enriched in amino acid metabolism pathways,among which tryptophan metabolism was identified as the core pathway.In conclusion,F.oxysporum infection accelerates quality deterioration and increases food safety risks in dried L.erythropterus fillets by dis-rupting physicochemical properties,amino acid metabolism,and flavor-related metabolic networks.Tryptophan metabolism plays a central regulatory role,and days 12-20 constitute a critical early-warning window for fungal deterioration.These find-ings provide a theoretical basis for fungal contamination control and preservation of dried aquatic products.关键词
红鱼干/尖孢镰孢菌/非靶代谢组学Key words
dried Lutjanus erythropterus fillet/Fusarium oxysporum/non-targeted metabolomics分类
农业科技引用本文复制引用
邓旗,范振煌,赵鹏,王庆文,肖晓玉,房志家,邱妹,M Kashif Imtiaz..尖孢镰孢菌侵染对红鱼干风味品质及双向代谢特征的影响[J].水产学报,2026,50(9):87-100,14.基金项目
国家自然科学基金(32302186) （32302186）
广东省教育厅重点领域专项(2025ZDZX4018) National Natural Science Foundation of China(32302186) （2025ZDZX4018）
Guangdong Provincial Department of Educa-tion Key Field Special Project(2025ZDZX4018) （2025ZDZX4018）
