肿瘤预防与治疗2026，Vol.39Issue(8)：615-624,10.DOI:10.3969/j.issn.1674-0904.2026.08.001
Claudin-18.2在胃癌中的表达及其生物学行为的研究
Expression of Claudin-18.2 in Gastric Cancer and the Protein's Biological Behavior
摘要
Abstract
Objective:To investigate the impact of tight junction protein 18.2(Claudin-18.2,CLDN18.2)overex-pression on malignant phenotypes,including proliferation,mi-gration,and invasion,in gastric cancer cells,and examine its expression in tumor tissues.Methods:Stable cell lines over-expressing human CLDN18.2 were established via lentiviral transduction in MFC and AGS cell lines.Cells transfected with the empty vector(Empty)served as controls.Protein expression was verified by Western blot and immunofluorescence.Cell proliferation was assessed using the CCK-8 assay and plate colony formation assay.Cell migration and invasion abilities were evaluated by Transwell assays.Additionally,the expression level of CLDN18.2 was detected in human gastric cancer tissue microarrays using immunofluorescence.Results:MFC and AGS cell lines stably overexpressing CLDN18.2 protein were suc-cessfully established,with CLDN18.2 localized to the cell membrane as confirmed by immunofluorescence.Compared with empty vector controls,CLDN18.2 overexpression significantly enhanced proliferation(MFC:from day 4 onward,day 4:P<0.05,day 5:P<0.01;AGS:days 3～5,all P<0.001).The colony formation rates increased to 21.07%vs 13.33%for MFC and 47.53%vs 36.53%for AGS(both P<0.05).The number of migrated cells increased to 1.56-fold for MFC and 1.53-fold for AGS(both P<0.01),and the number of invaded cells increased by 20.01%for MFC and 18.81%for AGS(both P<0.05).Human gastric cancer tissue microarray analysis revealed significantly higher CLDN18.2 expression in primary gastric cancer lesions than in paired adjacent gastric mucosa(P<0.01),with a dichotomous expression pattern observed in metastatic foci.Conclusion:CLDN18.2 overexpression promotes proliferation,colony formation,migration,and invasion of gastric cancer cells in vitro.Tissue microarray analysis validated its aberrant expression in gastric cancer tissues and its heterogeneous expression in metastatic foci.These findings support the potential of CLDN18.2 as a therapeutic target for anti-tumor treatment.关键词
Claudin-18.2/胃癌/细胞增殖/细胞迁移/细胞侵袭/靶向治疗Key words
Claudin-18.2/Gastric cancer/Cell proliferation/Cell migration/Cell invasion/Targeted therapy分类
医药卫生引用本文复制引用
刘小榕,谢华,朱亚杰,王悠,牟红梅,王绎,唐建宁,刘洋,寇玲娜,周进,魏阳..Claudin-18.2在胃癌中的表达及其生物学行为的研究[J].肿瘤预防与治疗,2026,39(8):615-624,10.基金项目
This study was supported by grants from Sichuan Medical Science and Technology Innovation Research As-sociation(No.YCH-KY-YCZD2024-031). 四川省医学科技创新研究会"医创之巅"专项科研项目(编号:YCH-KY-YCZD2024-031) （No.YCH-KY-YCZD2024-031）
