山东农业大学学报（自然科学版）2026，Vol.57Issue(4)：640-655,16.DOI:10.3969/j.issn.1000-2324.2026.04.006
营养元素添加对栀子根际土壤微生物多样性的影响
Microbial Diversity in the Rhizosphere Soil of Gardenia jasminoides under Different Nutrient Elements
摘要
Abstract
Rhizosphere microorganisms play a crucial role in plant growth and development,resistance to environmental stresses,and metabolic regulation.This study aims to clarify the effects of different concentrations of nitrogen(N),phosphorus(P),potassium(K),and magnesium(Mg)on rhizosphere soil microorganisms of Gardenia jasminoides seedlings.Four nutrient elements—N,P,K,and Mg—are selected,and the orthogonal experiment method is adopted.NH4NO3,KH2PO4,K2SO4 and MgSO4·7H2O are used to prepare different nutrient solutions with reference to Hoagland nutrient solution.Sampling is conducted 120 days after nutrient application to measure physiological indicators and active components.High-throughput sequencing technology is adopted to perform bioinformatics analysis on the microbial diversity and microbial community composition in its rhizosphere soil,respectively.The results show that based on the comprehensive ranking of principal component analysis(PCA),the optimal nutrient concentration combination for promoting the growth and physiology of gardenia seedlings is T8,consisting of 8 mmol/L NH4NO3,5 mmol/L KH2PO4,3 mmol/L K2SO4,8 mmol/L MgSO4·7H2O.The application of different nutrient elements reduces the abundance and diversity of the bacterial community in the rhizosphere soil of G.jasminoides,while increasing those of the fungal community.Among these treatments,T3 significantly enhances the abundance and diversity of the fungal community in the rhizosphere soil of G.jasminoides.Across all treatments,the dominant bacterial phyla in the rhizosphere soil of G.jasminoides are mainly Proteobacteria,Firmicutes,and Actinobacteria,and the dominant fungal phyla are scomycota,Basidiomycota,and Mucoromycota.Overall,the rational addition of nutrient elements can improve the composition and diversity of rhizosphere bacterial and fungal communities of G.jasminoides,providing new insights and theoretical support for the selection of fertilization strategies for G.jasminoides.关键词
营养元素/栀子幼苗/根际/土壤微生物Key words
Nutrient elements/Gardenia jasminoides/rhizosphere/soil microorganisms分类
农业科技引用本文复制引用
高宇欣,叶泽霖,覃兴化,徐易溱,高波,卢颐,张典铨,邹小兴..营养元素添加对栀子根际土壤微生物多样性的影响[J].山东农业大学学报（自然科学版）,2026,57(4):640-655,16.基金项目
2024年中央财政林业科技推广示范项目(闽[2024]TG22) （闽[2024]TG22）
福建省科技厅高校产学合作项目(2022N5009) （2022N5009）
邵武市二都国有林场有限责任公司资助项目,药赏两用栀子良种'分关1号'和'灵地珍栀'丰产栽培示范(KH250309A) （KH250309A）
