中国森林病虫2026，Vol.45Issue(4)：10-16,7.DOI:10.19688/j.cnki.issn1671-0886.20250071
云杉叶枯病生防菌株鉴定与筛选
Identification and screening of biocontrol strains for spruce leaf blight
摘要
Abstract
To screen for highly effective biocontrol strains against spruce leaf blight,six strains which exhibited antagonistic effects against the pathogen of spruce leaf blight,Nothophoma quercina,were isolated from the rhizosphere soil samples of healthy spruce trees.These strains were identified using morphological,physiological and biochemical characteristics as well as molecular biological methods.The antagonistic spectra of the six strains were obtained by dual-culture plate confrontation method.The control efficacy of the biocontrol strains was determined by in vitro experiments.The results showed that six strains were identified as Bacillus subtilis,which exhibited a broad-spectrum inhibitory effect.Six biocontrol strains were found to have good inhibitory effect on mycelial growth of N.quercina,the inhibitory rate was from 54.76%to 72.90%.The in vitro control efficacy of the bacterial strains was from 48.5%to 64.4%.Results provide beneficial biocontrol strains for the green prevention and control of spruce leaf blight.关键词
云杉叶枯病/筛选鉴定/生物防治/枯草芽孢杆菌/拟枝孢镰刀菌Key words
spruce leaf blight/screening and identification/biological control/Bacillus subtilis/Fusarium sporotrichioides分类
农业科技引用本文复制引用
王欣悦,王浙吉,戈素芬,韩娅茹,宋茁,夏博..云杉叶枯病生防菌株鉴定与筛选[J].中国森林病虫,2026,45(4):10-16,7.基金项目
国家自然科学基金项目"大连市公园云杉衰退现象分析及调控技术"(31300541) （31300541）
