时珍国医国药2026，Vol.37Issue(16)：3038-3045,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-1606
通窍平喘方调节TLR4/MyD88/NF-кB通路及免疫平衡改善过敏性鼻炎-哮喘综合征小鼠炎症反应
Tongqiao Pingchuan Formula(通窍平喘方)to ameliorate inflammatory responses in the model mice of combined allergic rhinitis and asthma syndrome by modulating the TLR4/MyD88/NF-κB signaling pathway and immune balance
摘要
Abstract
Objective To explore the effects and potential mechanisms of Tongqiao Pingchuan Formula(通窍平喘方,TQPCF)on a mouse model of combined allergic rhinitis and asthma syndrome(CARAS).Methods Thirty-six mice were randomly divided into the blank control,model control,positive control(dexamethasone,1.5 mg/kg/day),and TQPCF low/medium/high-dose groups(5.85,11.7,23.4 g/kg/day).Except for the blank group,mice were sensitized and challenged with OVA/Al(OH)3 to establish CARAS and then administered respective treatments.Observe the behavioral characteristics of each group of mice.Pathological changes in nasal mucosa and lung tissue were observed using HE and PAS staining.Levels of IL-4,IL-13,IFN-γ,IL-6,and TNF-α in bronchoalveolar lavage fluid(BALF)and nasal lavage fluid(NALF)were measured by ELISA.RT-qPCR detected mRNA levels of TLR4,MyD88,NF-κB p65,T-bet,and GATA3 in nasal mucosa and lung tissue.Western blot(WB)was used to assess protein levels of these targets in lung tissue.Results Model group mice showed an increase in behavioral scores,along with exhibited structural disruption,inflammatory cell infiltration,and goblet cell hyperplasia in nasal mucosa and lungs.In BALF and NALF,IL-4,IL-13,IL-6,and TNF-α levels were elevated,while IFN-γ was reduced.In nasal mucosa and lung tissue,TLR4,MyD88,NF-κB p65,and GATA3 mRNA expres-sions were increased,whereas T-bet mRNA was decreased.Similarly,TLR4,MyD88,NF-κB p65,and GATA3 protein levels in lung tissue were increased,and T-bet protein was decreased.Compared to the model group,TQPCF administration reduced the behavioral score,ameliorated pathological damage in nasal mucosa and lungs,decreased IL-4,IL-13,IL-6,and TNF-α levels in BALF and NALF,increased IFN-γ levels,and modulated the expression of TLR4,MyD88,NF-κB p65,GATA3,and T-bet at both mRNA and protein levels in nasal mucosa and lung tissue.Conclusion TQPCF alleviates allergic inflammatory responses in CARAS mice,poten-tially by inhibiting the TLR4/MyD88/NF-κB pathway and modulating Th1/Th2 immune balance.关键词
通窍平喘方/TLR4/MyD88/NF-кB/免疫平衡/过敏性鼻炎-哮喘综合征Key words
Tongqiao Pingchuan Formula(通窍平喘方)/TLR4/MyD88/NF-κB/Immune balance/Combined allergic rhinitis and asthma syndrome分类
医药卫生引用本文复制引用
王追越,刘亚尊,薛辉,薛征..通窍平喘方调节TLR4/MyD88/NF-кB通路及免疫平衡改善过敏性鼻炎-哮喘综合征小鼠炎症反应[J].时珍国医国药,2026,37(16):3038-3045,8.基金项目
国家自然科学基金面上项目(82274576) （82274576）
国家重点研发计划(2024YFC3505903 （）
2024YFC3505900) （）
上海市2022年度"科技创新行动计划"医学创新研究专项项目(22Y21920400) （22Y21920400）
