天津农业科学2026，Vol.32Issue(7)：32-39,44,9.DOI:10.3969/j.issn.1006-6500.2026.07.006
'云烟87'上部叶成熟进程中萜类香气前体物质的广泛靶向代谢组学分析
Widely Targeted Metabolomic Analysis of Terpenoid Aroma Precursors during the Maturation of Upper Leaves in'Yunyan 87'Tobacco
摘要
Abstract
To elucidate the metabolic patterns of terpenoid aroma precursors during the maturation process of upper leaves in fresh-flavor style flue-cured tobacco,upper leaves of'Yunyan 87'were used as the experimental material,and widely targeted metabolomics based on UPLC-MS/MS was applied to systematically analyze the dynamic changes of metabolites at the early(T1),middle(T2),and late(T3)stages of maturation.The analysis specifically focused on the differential accumulation and KEGG pathway enrichment of ter-penoid compounds.A total of 2 441 metabolites were identified,among which terpenoids accounted for the highest proportion(495 compounds,20.28%).During the maturation of upper leaves,terpenoids generally showed a trend of decreasing first and then increas-ing.From T1 to T2,the number of down-regulated terpenoids(90 metabolites)was higher than that of up-regulated ones(39 metabolites),and the differentially accumulated metabolites were significantly enriched in the carotenoid biosynthesis pathway.Con-versely,from T2 to T3,up-regulated terpenoids(46 metabolites)exceeded down-regulated ones(20 metabolites),with the DAMs pri-marily enriched in plant hormone signal transduction pathways,particularly the abscisic acid(ABA)-mediated senescence process.In conclusion,this study elucidates the metabolic shifts during the maturation of upper leaves in'Yunyan 87'and reveals that the T2 stage is a key metabolic turning point for the formation and transformation of terpenoid aroma precursors,providing important metabolomic evi-dence for the timely harvesting of upper leaves.关键词
烤烟上部叶/代谢组学/萜类香气前体物/差异代谢物/类胡萝卜素Key words
upper leaves of flue-cured tobacco/metabolomics/terpenoid aroma precursors/differential metabolites/carotenoids分类
农业科技引用本文复制引用
周喜新,王子陶,胡茂茂,邓智慧,邓羽翔,赵友根..'云烟87'上部叶成熟进程中萜类香气前体物质的广泛靶向代谢组学分析[J].天津农业科学,2026,32(7):32-39,44,9.基金项目
四川中烟工业有限公司科技项目(10202318BA500) （10202318BA500）
湖南省自然科学基金项目(2025JJ70317) （2025JJ70317）
