天津中医药大学学报2026，Vol.45Issue(7)：799-803,5.DOI:10.11656/j.issn.1673-9043.2026.07.06
超高效液相色谱-串联质谱法测定清音饮中5种有效成分含量研究
Simultaneous determination of five active components in Qingyin Yin by UPLC-MS/MS
摘要
Abstract
[Objective]To establish an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous determination of five bioactive components:aurantio-obtusin,chlorogenic acid,luteoloside,3,5-O-dicaffeoylquinic acid,and 4,5-O-dicaffeoylquinic acid in Qingyin Yin.[Methods]Chromatographic separation was performed on a Waters ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7 μm)with a gradient elution program using 0.1%formic acid in water(A)and acetonitrile(B)as the mobile phase at a flow rate of 0.3 mL/min.The column temperature was set at 35 ℃,and the injection volume was 5 μL.Mass spectrometric detection was carried out in negative electrospray ionization(ESI)mode with multiple reaction monitoring(MRM).[Results]The method was validated and showed good linearity for all five components within their respective concentration ranges,with correlation coefficients(r)not less than 0.998 7.The precision,repeatability,and stability of the method were satisfactory.The average recoveries ranged from 94.00%to 112.55%.[Conclusion]The established UPLC-MS/MS method is sensitive,specific,and reproducible,providing a reliable approach for the quality control of Qingyin Yin.关键词
清音饮/超高效液相色谱-串联质谱法/化学成分/含量测定Key words
Qingyin Yin/UPLC-MS/MS/chemical component/content determination分类
医药卫生引用本文复制引用
李宇,何畅,王瑞,范栢爽,陈涛,柴士伟,王洋..超高效液相色谱-串联质谱法测定清音饮中5种有效成分含量研究[J].天津中医药大学学报,2026,45(7):799-803,5.基金项目
天津市南开区中医药传承创新发展示范试点项目(20240204005) （20240204005）
2025年度中医药监测统计研究课题项目(2025JCTJC120). （2025JCTJC120）
