天津中医药大学学报2026，Vol.45Issue(7)：821-829,9.DOI:10.11656/j.issn.1673-9043.2026.07.09
东革阿里叶提取物调节肾脏尿酸转运体改善高尿酸血症作用研究
Eurycoma longifolia jack leaf extract alleviates hyperuricemia by regulating renal urate transporters
摘要
Abstract
[Objective]To investigate the therapeutic efficacy and underlying mechanisms of Eurycoma longifolia jack leaf extract(ELL)in regulating hyperuricemia.[Methods]A mouse model of hyperuricemia was established via continuous 3-week co-administration of potassium oxonate and adenine.Serum uric acid(SUA)and creatinine levels were monitored weekly.Mice were placed in metabolic cages 24 h before sacrifice to collect urine for uric acid quantification and calculation of uric acid clearance(Cur).Renal histopathology was assessed by HE staining.RT-PCR and Western blot were employed to analyze the expression of urate transporter-related genes and proteins in kidney tissues.[Results]Progressive hyperuricemia led to decreased body weight,reduced food intake,and increased water consumption compared with normal controls.ELL at a dose of more than 200 mg/kg restored weight gain.After 1 week of modeling,SUA levels surged,accompanied by increased urine volume but decreased uric acid excretion.Cur declined significantly over time.ELL treatment markedly reduced SUA and serum creatinine while elevating Cur and creatinine clearance rate(Ccr).HE staining revealed that ELL alleviated renal pathological damage and improved kidney function.Molecular analyses demonstrated that ELL downregulated renal URAT1(urate-anion transporter 1)and GLUT9(glucose transporter 9)expression while upregulating OAT1(organic anion transporter 1),NPT1(Na-dependent phosphate cotransporter 1),and ABCG2(ATP-binding cassette superfamily G member 2).[Conclusion]ELL lowered SUA levels in hyperuricemia mice by enhancing Cur and Ccr and protected renal tissue.The mechanism involved dual modulation of urate transporters:inhibiting reabsorption via downregulation of URAT1/GLUT9 and promoting excretion via upregulation of NPT1/OAT1/ABCG2.关键词
东革阿里叶提取物/高尿酸血症/尿酸盐转运体/肾脏排泄Key words
Eurycoma longifolia jack leaf extract/hyperuricemia/urate transporter/renal excretion分类
医药卫生引用本文复制引用
李思一,潘具洁,温少诗,包瑞霞,车梓瑜,王丹,王涛..东革阿里叶提取物调节肾脏尿酸转运体改善高尿酸血症作用研究[J].天津中医药大学学报,2026,45(7):821-829,9.基金项目
国家自然科学基金项目(82304870) （82304870）
天津市卫生健康委员会项目(2024009) （2024009）
大学生创新创业训练计划项目(202510063002). （202510063002）
