现代中西医结合杂志2026，Vol.35Issue(13)：1761-1766,1772,7.DOI:10.3969/j.issn.1008-8849.2026.13.002
基于氧化应激-SHP2/PI3K通路探讨植物乳植杆菌LP45代谢产物对间充质干细胞的影响
Lactobacillus plantarum LP45 metabolites promote the proliferation,migration and differentiation of mesenchymal stem cells via oxidative stress-SHP2/PI3K pathway
摘要
Abstract
Objective It is to the effect of Lactobacillus plantarum LP45 on the proliferation,migration and differentia-tion of mesenchymal stem cells,and to explore its mechanism of action based on oxidative stress-src homology 2 domain-containing protein tyrosine phosphatase 2(SHP2)/phosphoinositide 3-kinase(PI3K)pathway.Methods Rat bone marrow mesenchymal stem cells in logarithmic growth phase were taken for the experiment.The control group was normally cul-tured,the LPS group was cultured with LPS,and the LP45 group was cultured with LPS and LP45 supernatant.The cell proliferation was detected by CCK-8 assay,the cell migration was detected by Transwell assay,the levels of superoxide dis-mutase(SOD)and malondialdehyde(MDA)in the cells were measured by ELISA,and the protein expressions of nicotin-amide adenine dinucleotide phosphate oxidase(NOX)2,NOX4,p-SHP2,p-PI3K,SRY-related high-mobility protein transcription factor 9(SOX9),and type Ⅱ collagen(Col Ⅱ)in the cells were detected by Western blot.Results Com-pared with the control group,the cell proliferation absorbance value and the number of migrating cells were significantly re-duced in the LPS group(both P<0.05);the level of MDA and the relative protein expressions of NOX2 and NOX4 in the cells were all significantly increased(all P<0.05),while the level of SOD and the relative protein expressions of p-SHP2,p-PI3K,SOX9 and Col Ⅱ in the cells were all significantly reduced(all P<0.05).Compared with the LPS group,the cell proliferation absorbance value and the number of migrating cells were significantly increased in the LP45 group(both P<0.05);the level of MDA and the relative protein expressions of NOX2 and NOX4 in the cells were all significantly de-creased(all P<0.05),while the level of SOD and the relative protein expressions of p-SHP2,p-PI3K,SOX9 and Col Ⅱin the cells were all significantly increased(all P<0.05).Conclusion Lactobacillus plantarum LP45 metabolites can in-hibit the inhibiting effect of oxidative stress on the SHP2/PI3K signaling pathway and promote the proliferation,migration and differentiation of mesenchymal stem cells.关键词
植物乳植杆菌LP45/氧化应激/Src同源2结构域的蛋白酪氨酸磷酸酶2/磷脂酰肌醇3-激酶/间充质干细胞Key words
Lactobacillus plantarum LP45/oxidative stress/SHP2/PI3K/mesenchymal stromal cells分类
医药卫生引用本文复制引用
闫正,郑海蓉,宋世婧,李明霞,张婷婷,张伟,路江浩,贾晓蒙,雷敏..基于氧化应激-SHP2/PI3K通路探讨植物乳植杆菌LP45代谢产物对间充质干细胞的影响[J].现代中西医结合杂志,2026,35(13):1761-1766,1772,7.基金项目
2022年政府资助临床医学优秀人才培养项目(29) （29）
河北省卫生和计划生育委员会重点科技研究计划项目(20240560) （20240560）
2025年度河北医科大学第三医院院内中医药、中西医结合课题(ydsyzyy202504) （ydsyzyy202504）
