烟草科技2026，Vol.59Issue(8)：1-8,8.DOI:10.16135/j.issn1002-0861.2025.0638
基于细胞的卷烟烟气中主要甜感物质的甜度值测定
Cell-based quantification of sweetness for key sweet substances in cigarette smoke
摘要
Abstract
To overcome the limitations of current sweetness measurement methods for cigarette smoke and achieve objective quantification of sweet sensory sensation,HEK293 cell lines with the overexpression of human sweet taste receptor T1R2/T1R3 were established.By employing calcium ion imaging technology to monitor real-time changes in intracellular calcium signals induced by sweet substances,quantitative characterization of sweetness response was realized.Using this technique,sweetness values of ten previously reported representative sweet substances in cigarette smoke including furfural and furanone were systematically determined.Furthermore,the influence of the total particulate matter(TPM)on the sweetness performance of these sweet substances was investigated.The results showed that:1)Most sweet substances exhibited varying degrees of reduced sweetness values in TPM matrix,which may be attributed to the competitive or non-competitive inhibition on the sweet receptors by other complex smoke components.2)Partial substances(fenugreek lactone and 3,5-dimethyl-1,2-cyclopentanedione)maintained stable or slightly enhanced sweetness in the TPM matrix,suggesting that the smoke matrix exerted differential effects on sweetness of different sweet substances and might even involve synergistic effects.These findings suggest that cigarette flavor regulation should focus on components that exhibit stable or enhanced sweetness in the smoke matrix.关键词
卷烟烟气/甜感物质/甜度值/人源甜味受体T1R2/T1R3/钙离子成像/定量分析Key words
Cigarette smoke/Sweet substance/Sweetness value/Human sweet taste receptor T1R2/T1R3/Calcium imaging/Quantitative analysis分类
轻工纺织引用本文复制引用
马铖杰,颜权平,谢复炜,聂聪,华辰凤,张晓兵,赵阁..基于细胞的卷烟烟气中主要甜感物质的甜度值测定[J].烟草科技,2026,59(8):1-8,8.基金项目
中国烟草总公司科技项目"基于类器官仿生模型的烟气风格定量表征技术研究"(322023AA0290)、"甜味受体正变构调节剂对烟气甜味感受的增强作用研究"(352024AA0600) （322023AA0290）
郑州烟草研究院青年人才托举工程计划项目"基于基因敲除技术的烟气甜味定量表征方法研究"(312024CR0020). （312024CR0020）
