烟草科技2026，Vol.59Issue(8)：24-36,13.DOI:10.16135/j.issn1002-0861.2025.0523
烘烤过程中变黄期湿球温度对翠碧一号烟叶品质与代谢物转化的影响
Effects of wet bulb temperature on quality and metabolite transformation of Cuibi 1 tobacco leaves during yellowing stage of curing process
摘要
Abstract
To investigate the effects of wet bulb temperature during the yellowing stage of curing process on the quality and metabolite transformation of tobacco leaves,middle leaves of Cuibi 1(CB-1)were studied and the wet bulb temperatures for the early,middle and late yellowing stages were set as 37.0-37.5℃,36.0-37.0℃,36.0-37.0℃(T1,low tempreture and humidity treatment),37.5-38.0℃,38.0-39.0℃,38.0℃(T2,middle tempreture and humidity treatment),and 38.0℃,39.0-40.0℃,36.0-37.0℃(T3,high tempreture and humidity treatment).The chemical composition and sensary quality of the cured tobacco leaves were evaluated,and the differential metabolites between the three curing treatments at the yellowing and color-fixing stages were identified using non-targeted metabolomics techniques.The results showed that:1)Compared with T1 and T3,T2 yielded the most favourable index values,including the contents of nicotine,total sugar,reducing sugar,chloride ions and sugar-alkali ratio,chlor-alkali ratio and potassium-chloride ratio.It also achieved the highest scores for sensory style and quality characteristics,with a more pronounced flavor style and superior freshness,sweetness and aroma quality,and the best overall sensory score.2)Metabolomics analysis identified 4,327 metabolites in CB-1 tobacco leaves across the three curing treatments.From the middle and later yellowing stage to the middle color-fixing stage,more than 2,000 differential metabolites were identified among the three treatments.The greatest number of differential metabolites was observed at the later yellowing stage,indicating that wet bulb temperature at this stage was critical for the quality formation.Significant changes were observed in differenrtial metabolites including organic nitrogen compounds,carbohydrates,flavonoids,alkaloids,terpenes and lipids during the curing process.3)Compared with T1 and T3,T2 exhibited more complete conversion of organic nitrogen compounds at the later color-fixing stage.Carbohydrate decomposition produced more sweet substances such as melibiose,catalpol,galacturonic acid,L-gulose and mannotriose,contributing to the fresh and sweet flavor style of tobacco leaves under T2 treatment.Under T2 treatment,the relative abundances of glabridin,betanin,aconine,and acetylvalerenolic acid were high,and the aroma style and characteristics were more distinctive.Furthermore,the relative abundances of reserpine,oripavine,baptifoline,ergotamine and α-narcotine at the later yellowing stage were relatively high under T2 treatment.关键词
翠碧一号/中部烟叶/烘烤/湿球温度/代谢物Key words
Cuibi 1/Middle tobacco leaf/Curing/Wet bulb temperature/Metabolite分类
轻工纺织引用本文复制引用
王新旺,张时旭,李文卿,张炳辉,刘光菊,杜昌阳,李兆伟..烘烤过程中变黄期湿球温度对翠碧一号烟叶品质与代谢物转化的影响[J].烟草科技,2026,59(8):24-36,13.基金项目
中国烟草总公司福建省公司科技计划项目"烟叶采收与烘烤过程内在主要物质代谢组学研究"(2023350000200119). （2023350000200119）
