烟草科技2026，Vol.59Issue(8)：49-56,65,9.DOI:10.16135/j.issn1002-0861.2025.0574
烟用料液贮存中微生物群落演替及山梨酸钾对致腐菌的抑制效果
Microbial community succession during the storage of tobacco casing and the inhibitory effect of potassium sorbate against spoilage microorganisms
摘要
Abstract
To clarify microbial community succession and the inhibitory effect of potassium sorbate on the dominant spoilage microorganisms of tobacco casing during storage,metagenomics and molecular biological technologies were employed to systematically investigate the dynamic changes in microbial community,identify the dominant spoilage microorganism,elucidate its biological characteristics,and further evaluate the inhibitory effects of potassium sorbate against the strain.The results showed that on the 7th day of storage,the total viable count and yeast count in the tobacco casing increased by 4.7×103 and 2.8×103 CFU/mL,respectively,compared to those on the 5th day,accompanied by the emergence of sour odor,gas production and the formation of a yellow microbial film.Within 0-5 days of storage,the richness and diversity of microbial species in tobacco casing showed an upward trend but a significantly decrease on day 7,with reduced community uniformity.The microbial community structure was dominated by a limited number of species,with Wickerhammyces gradually becoming the dominant bacterial group.The dominant spoilage microorganism was identified as Wickerhamomyces anomalus.The optimal growth conditions for this strain were 30℃with a pH value of 6.0,and the best carbon sources were sucrose,fructose,and glucose.The antibacterial results revealed that the inhibitory effect of potassium sorbate on W.anomalus exhibited concentration dependence.At a concentration of 1.00 mg/mL,a significant delay in the growth of the strain was observed.As the concentration gradient of potassium sorbate increased,the growth of the strain gradually stagnated,and the minimum inhibitory concentration(MIC)was determined to be 8.00 mg/mL.关键词
烟用料液/致腐菌/微生物群落/生物学特性/山梨酸钾Key words
Tobacco casing/Spoilage microorganism/Microbial community/Biological characteristics/Potassium sorbate分类
轻工纺织引用本文复制引用
刘前进,张洁,蔡莉莉,文卓,张峻松,臧翔宇,李甜甜,巩笑楠,杨柳依,李耀光,陈彪,马胜涛,张靖楠,万帅..烟用料液贮存中微生物群落演替及山梨酸钾对致腐菌的抑制效果[J].烟草科技,2026,59(8):49-56,65,9.基金项目
河南中烟工业有限责任公司科技项目"烟用加料香精致腐微生物解析及控制技术应用研究"(AW2024008). （AW2024008）
