云南化工2026，Vol.53Issue(8)：18-22,5.DOI:10.3969/j.issn.1004-275X.2026.08.05
响应面优化黄精多糖超声辅助复合酶提取工艺与活性分析
Optimization of Ultrasound-assisted Complex Enzyme Extraction of Polysaccharides from Polygonatum Sibiricum Using Response Surface Methodology and Evaluation of Its Antioxidant Activity
摘要
Abstract
To promote the deeper exploitation of Polygonatum sibiricum polysaccharides in pharmaceutical and food fields,the ultrasound-assis-ted complex enzyme extraction of polysaccharides from Polygonatum sibiricum sourced from Fanjing Mountain was optimized via response surface methodology,followed by an assessment of its biological activity.The results indicated that the optimal extraction conditions were as follows:com-plex enzyme concentration of 6%,ultrasound-assisted enzymolysis time of 37 min,and ethanol precipitation time of 46 min.Under the condi-tions,the polysaccharides content reached 47.72%and the polysaccharides demonstrated good antioxidant activity with DPPH radical scavenging rates of 54.18%and ABTS radical scavenging rates of 32.77%at a concentration of 8 mg/mL.关键词
黄精多糖/超声/复合酶/抗氧化活性Key words
Polygonatum Sibiricum of Polysaccharides/Ultrasound/Compound Enzyme/Antioxidant Activity分类
轻工纺织引用本文复制引用
唐萍,李佳丽,朱艳,冯祝婷..响应面优化黄精多糖超声辅助复合酶提取工艺与活性分析[J].云南化工,2026,53(8):18-22,5.基金项目
铜仁市科学技术局基础研究计划[铜市科研(2022)52号] （2022）
贵州健康职业学院自然科学课题(2022zk05). （2022zk05）
