云南民族大学学报（自然科学版）2026，Vol.35Issue(4)：450-455,471,7.DOI:10.3969/j.issn.1672-8513.2026.04.002
野坝子总黄酮提取工艺及抗氧化活性研究
Study on the extraction technology and antioxidant activity of total flavonoids from the foliage of Elsholtzia rugulosa Hemsl.
摘要
Abstract
This study aims to investigate the extraction methods and antioxidant activity of total flavonoids from Elsholtzia rugulosa Hemsl.foliage.The extraction process for total flavonoids from Elsholtzia rugulosa Hemsl.foliage was optimized using an ultrasound-assisted method based on single-factor experiments combined with response surface methodology,and their antioxidant activity was systematically evaluated.The results indicate that the optimal extraction conditions for total flavonoids are extraction time 25 min,liquid-to-solid ratio 15 mL/g,and ethanol volume fraction 80％.Under these conditions,the extraction yield of total flavonoids reached 5.71％.The extract exhibited excellent antioxidant activity,with IC50 values of 43.707 μg/mL for DPPH radical scavenging capacity and 54.012 μg/mL for ABTS radical scavenging capacity.These findings provide essential process parameters and a theoretical foundation for the development of E.rugulosa Hemsl.in functional foods and natural antioxidant applications.关键词
野坝子叶/超声波提取/总黄酮/响应面分析法/抗氧化Key words
Elsholtzia rugulosa Hemsl/ultrasonic extraction/total flavonoids/response surface methodology/antioxidant activity分类
医药卫生引用本文复制引用
蔡安明,朱红亮,饶洪穗,吴思静,温敏,袁燕..野坝子总黄酮提取工艺及抗氧化活性研究[J].云南民族大学学报（自然科学版）,2026,35(4):450-455,471,7.基金项目
国家自然科学基金(32360226) （32360226）
云南民族大学审核评估教育教学研究与实践项目(2022JYJX-06) （2022JYJX-06）
云南民族大学教育教学改革研究委托项目(2023JYJXGGWT-57) （2023JYJXGGWT-57）
云南省2024年大学生创新训练计划项目(934). （934）
