云南民族大学学报（自然科学版）2026，Vol.35Issue(4)：514-523,10.DOI:10.3969/j.issn.1672-8513.2026.04.009
千层纸素A对胃癌SGC-7901细胞的影响及其调控铁死亡机制研究
Effects of oroxylin A on gastric cancer SGC-7901 cells and its regulation of ferroptosis mechanism
摘要
Abstract
The study investigated the effects and mechanisms of oroxylin A on gastric cancer SGC-7901 cells.The methods employed included cell viability assay kit,Hoechst 33258 fluorescent probe,cell scratch assay,Transwell assays,reactive oxygen species(ROS)fluorescent probe,JC-1 fluorescent probe,ferrous ion(Fe2+)fluorescent probe,network pharmacology,and Western blot to explore the impact of oroxylin A on cell viability,apoptosis,migration,invasion,and ferroptosis,as well as its regulatory mechanisms of ferroptosis in SGC-7901 cells.The results revealed that oroxylin A inhibited SGC-7901 cell viability,scratch healing,migration,and invasion,as well as induced apoptosis,ROS accumulation,mitochondrial membrane potential depolarization,and Fe2+levels.Additionally,oroxylin A reduced the expression of the epidermal growth factor receptor(EGFR)protein in cells.The EGFR agonist NSC 228155 can reverse the increase in Fe2+levels and the decrease in cell viability induced by oroxylin A.The results indicate that oroxylin A exhibits a clear anticancer effect on gastric cancer SGC-7901 cells and induces cellular ferroptosis through the inhibition of EGFR.关键词
千层纸素A/胃癌/SGC-7901细胞/增殖/迁移/侵袭/铁死亡/EGFRKey words
oroxylin A/gastric cancer/SGC-7901 cell/proliferation/migration/invasion/ferroptosis/EGFR分类
医药卫生引用本文复制引用
王明西,夏芸,倪萍..千层纸素A对胃癌SGC-7901细胞的影响及其调控铁死亡机制研究[J].云南民族大学学报（自然科学版）,2026,35(4):514-523,10.基金项目
南京市卫生科技发展专项(YKK20210). （YKK20210）
