亚太传统医药2026，Vol.22Issue(8)：24-31,8.DOI:10.11954/ytctyy.202608004
如意金黄散通过自噬途径调控NLRP3炎症小体表达治疗皮肤脓肿的机制研究
Mechanistic Investigation of Ruyi Jinhuang Powder in Regulating NLRP3 Inflammasome Via the Autophagy Pathway for the Treatment of Skin Abscess
摘要
Abstract
Objective:Establish a mouse model of skin abscess infected with Staphylococcus aureus and evaluate the in-tervention effect and mechanism of Ruyi Jinhuang Powder on autophagy and NOD-like receptor protein 3(NLRP3 in-flammasome)expression in mice with skin abscess.Methods:Using hematoxylin eosin(HE)staining method to ob-serve the histopathological morphology of tissues;Observation of autophagosome formation under transmission elec-tron microscopy;Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of IL-18 and TNF-α in tissues;Western blot and immunofluorescence staining were used to detect the protein expression of Beclin1,LC3 Ⅱ/Ⅰ,and NLRP3 in tissues.Results:The HE staining results showed that compared with the Sham group,the skin structure of the model group was severely abnormal,with no visible epidermal structure and exposed dermis layer(red arrow).There were a large number of inflammatory cells infiltrating the tissue(green arrow),and granulation tissue formation was observed locally;The levels of IL-18 and TNF-α in mouse serum were significantly upregulated(P<0.001,P<0.001);Western blot and immunofluorescence detection both showed significant increases in Beclin1,LC3Ⅱ/Ⅰ,and NLRP3 levels(P<0.05,P<0.05,P<0.001).After 10 days of intervention with Ruyi Jinhuang Powder,compared with the model group,the skin structure of the Model+RYHJS group improved,the degree of tissue inflam-matory cell infiltration was relatively reduced,and a small amount of granulation tissue was observed locally;Transmis-sion electron microscopy observation showed that after intervention with Ruyi Jinhuang Powder,an increase in the pro-portion of autophagosomes was observed in the skin tissue of mice;The levels of IL-18 and TNF-α in serum were sig-nificantly downregulated(P<0.05,P<0.001);Western blot and immunofluorescence detection both suggest that in-tervention with Ruyi Jinhuang Powder can significantly reduce the expression of NLRP3 protein(P<0.001)and sig-nificantly increase the expression levels of autophagy related genes Beclin1 and LC3 Ⅱ/Ⅰ(P<0.001,P<0.05).Af-ter administering 3-MA,both Western blot and immunofluorescence detection methods showed a significant increase in NLRP3 expression(P<0.01).Conclusion:Studies suggest that Ruyi Jinhuang Powder may exert therapeutic effects against skin abscesses by suppressing NLRP3 inflammasome signaling through the autophagy pathway.关键词
如意金黄散/自噬/NLRP3/脓肿Key words
Ruyi Jinhuang Powder/autophagy/NLRP3/abscess分类
医药卫生引用本文复制引用
王重阳,刘首记,马瑛,王姝理,谢敏,曾娟妮..如意金黄散通过自噬途径调控NLRP3炎症小体表达治疗皮肤脓肿的机制研究[J].亚太传统医药,2026,22(8):24-31,8.基金项目
湖南省教育厅科学研究项目(24C1178) （24C1178）
