医学信息2026，Vol.39Issue(15)：1-11,11.DOI:10.3969/j.issn.1006-1959.2026.15.001
氧化磷酸化相关基因在骨关节炎免疫微环境中的生物信息学分析
Bioinformatics Analysis of Oxidative Phosphorylation-related Genes in the Immune Microenvironment of Osteoarthritis
摘要
Abstract
Objective To investigate the role of oxidative phosphorylation-related genes in the pathogenesis of osteoarthritis and their relationship with the immune microenvironment.Methods Based on osteoarthritis synovial tissue datasets(GSE55235 and GSE55457)from Gene Expression Omnibus(GEO)database,differentially expressed genes related to oxidative phosphorylation were screened through differential expression analysis,and their mechanisms were explored using functional enrichment(GO/KEGG),immune infiltration(ssGSEA/CIBERSORT),and regulatory network(PPI,miRNA-TF-RBP)analysis.Results Fifteen differentially expressed genes related to oxidative phosphorylation were identified,which were significantly enriched in pathways such as mitochondrial respiratory chain complex assembly and oxidative phosphorylation(P<0.05).Immune infiltration analysis revealed significantly elevated levels of 16 immune cell types in the osteoarthritis group(P<0.05);correlation analysis indicated that TWF2 was positively correlated with macrophage infiltration(r=0.69),while UCP2 was negatively correlated with resting CD4 memory T cells(r=-0.67).The regulatory network revealed interactions among 8 core oxidative phosphorylation genes,which were modulated by 25 miRNAs,27 TFs,and 20 RBPs.Conclusion Oxidative phosphorylation-related genes(TWF2,UCP2)drive the pathological progression of osteoarthritis by regulating macrophage polarization and T cell metabolic reprogramming,thereby inducing immune microenvironment imbalance.关键词
骨关节炎/氧化磷酸化/免疫浸润/生物信息学Key words
Osteoarthritis/Oxidative phosphorylation/Immune infiltration/Bioinformatics分类
医药卫生引用本文复制引用
张弘韬,张诚恺,赵振,牛舜..氧化磷酸化相关基因在骨关节炎免疫微环境中的生物信息学分析[J].医学信息,2026,39(15):1-11,11.基金项目
陕西省自然科学基础研究计划(一般项目-青年项目)(编号:2025JC-YBQN-1296) （一般项目-青年项目）
